Hexokinase 1 Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1726
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Hexokinase 1
Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IP, IF,
IHC, ICC, FC
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H, M, R
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102.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:2,000
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1:10-1:50
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1:50-1:200
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1:50-1:200
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1:50-1:200
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1:50-1:100
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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Hexokinase 1
Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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3098(Human);
15275(Mouse); 25058(Rat)
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SwissProt
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P19367(Human);
P17710(Mouse); P05708(Rat)
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Synonyms
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Hexokinase-1;
Brain form hexokinase; Hexokinase type I; HK I; HK1
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Category
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Metabolism
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Background
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The
hexokinases utilize Mg-ATP as a phosphoryl donor to catalyze the first step
of intracellular glucose metabolism, the conversion of glucose to
glucose-6-phosphate. Four hexokinase isoenzymes have been identified,
including hexokinase I (HXK I), hexokinase II (HXK II), hexokinase III (HXK
III) and hexokinase IV (HXK IV, also designated glucokinase or GCK).
Hexokinases I-III each contain an N-terminal cluster of hydrophobic amino
acids. Glucokinase lacks the N-terminal hydrophobic cluster. The hydrophobic
cluster is thought to be necessary for membrane binding. This is
substantiated by the finding that glucokinase has lower XYfinity for glucose
than do the other hexokinases. HXK I has been shown to be expressed in brain,
kidney and heart tissues as well as in hepatoma cell lines. HXK II is
involved in the uptake and utilization of glucose by adipose and skeletal
tissues. Of the hexokinases, HXK III has the highest XYfinity for glucose.
Glucokinase is expressed in pancreatic beta cells where it functions as a
glucose sensor, determining the “set point” for insulin secretion.
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包装清单:
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产品编号
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产品名称
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包装
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XY1726
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Hexokinase 1
Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
Hexokinase 1
Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10