Myosin light chain kinase Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1678
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Myosin light
chain kinase Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IF, IHC,
ICC, FC
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H, M, R
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110.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:2,000-1:10,000
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-
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1:100-1:500
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1:100-1:500
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1:100-1:500
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1:50-1:100
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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Myosin light
chain kinase Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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4638(Human);
117558(Mouse); 117558(Rat)
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SwissProt
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Q15746(Human);
Q6PDN3(Mouse); P20689(Rat)
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Synonyms
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MLCK; smMLCK;
Kinase-related protein; KRP; Telokin; MYLK; MLCK1; MYLK1
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Category
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Cytoskeletal
Signaling
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Background
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MLCK, a member
of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent
enzyme responsible for smooth muscle contraction via phosphorylation of a
specific serine in the N-terminus of myosin light chains (MLC), an event that
facilitates myosin interaction with actin filaments. It is a central
determinant in the development of vascular permeability and tissue edema
formation. In the nervous system it has been shown to control the growth
initiation of astrocytic processes in culture and to participate in
transmitter release at synapses formed between cultured sympathetic ganglion
cells. MLCK acts as a critical participant in signaling sequences that result
in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed
in a wide variety of adul and fetal tissues and in cultured endothelium with
qualitative expression appearing to be neither tissue- nor
development-specific. Non-muscle isoform 2 is the dominant splice variant
expressed in various tissues. The Telokin isoform, which binds calmodulin,
has been found in a wide variety of adul and fetal tissues. MLCK is probably
down-regulated by phosphorylation. The protein contains 1 fibronectin type
III domain and 9 immunoglobulin-like C2-type domains.
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包装清单:
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产品编号
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产品名称
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包装
|
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XY1678
|
Myosin light
chain kinase Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
Myosin light
chain kinase Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10