mTOR Rabbit Monoclonal Antibody
|
产品编号
|
产品名称
|
产品包装
|
产品价格
|
|
XY1648
|
mTOR Rabbit
Monoclonal Antibody
|
50μl
|
618.00元
|
|
来源
|
用途
|
交叉反应性
|
分子量
|
|
Rabbit
|
WB, IP, IHC
|
H, M, R
|
289.0kDa
|
WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
|
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
|
1:1,000
|
1:10-1:50
|
-
|
1:50-1:200
|
|
-
|
-
|
-
|
抗体详细信息如下:
|
About this
Antibody
|
|
Name
|
mTOR Rabbit
Monoclonal Antibody
|
|
Category
|
Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
|
|
Isotype
|
IgG
|
|
Purification
|
XYfinity
purification
|
|
About the
Immunogen
|
|
Immunogen
|
Recombinant
protein
|
|
Gene ID
|
2475(Human);
56717(Mouse); 56718(Rat)
|
|
SwissProt
|
P42345(Human);
Q9JLN9(Mouse); P42346(Rat)
|
|
Synonyms
|
FRAP; FRAP1;
FRAP2; RXYT1; RAPT1
|
|
Category
|
PI3K/Akt/mTOR
|
|
Background
|
The
phosphatidylinositol kinase (PIK) family members fall into two distinct
subgroups. The first subgroup contains proteins such as the PI 3- and PI
4-kinases and the second group comprises the PIK-related kinases. The
PIK-related kinases include Atm, DNA-PKCS and FRAP. These proteins have in
common a region of homology at their carboxy-termini that is not present in
the PI 3- and PI 4-kinases. The Atm gene is mutated in the autosomal
recessive disorder ataxia telangiectasia (AT) that is characterized by cerebellar
degeneration (ataxia) and the appearance of dilated blood vessels
(telangiectases) in the conjunctivae of the eyes. AT cells are hypersensitive
to ionizing radiation, impaired in mediating the inhibition of DNA synthesis
and display delays in p53 induction. DNA-PK is a heterotrimeric DNA binding
enzyme that is composed of a large subunit, DNA-PKCS, and two smaller
subunits collectively known as Ku. The loss of DNA-PK leads to defects in DSB
repair and V(D)J recombination. FRAP can autophosphorylate on serine and bind
to Rapamycin/FKBP. FRAP is also an upstream regulator of S6 kinase and has
been implicated in the regulation of p27 and p21 expression.
|
包装清单:
|
产品编号
|
产品名称
|
包装
|
|
XY1648
|
mTOR Rabbit
Monoclonal Antibody
|
50μl
|
|
XY050
|
Western一抗稀释液
|
50ml
|
|
-
|
说明书
|
1份
|
保存条件:
mTOR Rabbit
Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10