AMPK alpha 1 Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1627
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AMPK alpha 1
Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IP, IF,
ICC, FC
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H, M, R
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63.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:5,000
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1:10-1:50
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1:50-1:200
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-
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1:50-1:200
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1:50-1:100
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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AMPK alpha 1
Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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5562(Human);
105787(Mouse); 65248(Rat)
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SwissProt
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Q13131(Human);
Q5EG47(Mouse); P54645(Rat)
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Synonyms
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PRKAA1; AMPK;
AMPKa1
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Category
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Cell Cycle
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Background
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AMPK (for
5'-AMP-activated protein kinase) is a heterotrimeric complex com-prising a
catalytic α subunit and regulatory β and γ subunits. It protects cells from stresses that cause ATP
depletion by switching off ATP-consuming bio-synthetic pathways. AMPK is
activated by high AMP and low ATP through a mechanism involving allosteric
regulation, promotion of phosphorylation by an upstream protein kinase known
as AMPK kinase, and inhibition of dephosphorylation. Activated AMPK can
phosphorylate and regulate in vivo hydroxy-methylglutaryl-CoA reductase and
acetyl-CoA carboxylase, which are key regulatory enzymes of sterol synthesis
and fatty acid synthesis, respectively. The human AMPKα1 and AMPKα2 genes encode 548 amino acid
and 552 amino acid proteins, respectively. Human AMPKβ1 encodes a 271 amino acid protein and human AMPKβ2 encodes a 272 amino acid protein. The human AMPKγ1 gene encodes a 331 amino acid protein. Human AMPKγ2 and AMPKγ3, which are 569 and 492 amino
acid proteins, respectively, contain unique N-terminal domains and may
participate directly in the binding of AMP within the AMPK complex.
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包装清单:
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产品编号
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产品名称
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包装
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XY1627
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AMPK alpha 1
Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
AMPK alpha 1
Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10