Cyclin B1 Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1606
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Cyclin B1
Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IP, IF,
IHC, ICC
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H
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55.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:5,000
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1:10-1:50
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1:50-1:200
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1:50-1:200
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1:50-1:200
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-
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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Cyclin B1
Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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891(Human)
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SwissProt
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P14635(Human)
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Synonyms
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CCNB
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Category
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Cell Cycle
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Background
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In eukaryotic
cells, mitosis is initiated following the activation of a protein kinase
known variously as maturation-promoting factor, M-phase specific histone
kinase or M-phase kinase. This protein kinase is composed of a catalytic
subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight
subunit (p13-Suc 1). The Cdc/cyclin enzyme is subject to multiple levels of
control, of which the regulation of the catalytic subunit by tyrosine
phosphorylation is the best understood. Tyrosine phosphorylation inhibits the
Cdc2/cyclin B enzyme; tyrosine dephosphorylation, occurring at the onset of
mitosis, directly activates the pre-MPF complex. Evidence has established
that B type cyclins not only act on M-phase regulatory subunits of the Cdc2
protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine
phosphatase, of which Cdc2 is the physiological substrate. The specificity of
this effect is shown by the inability of either cyclin A or cyclin D1 to
display any such stimulation of Cdc25A or Cdc25B.
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包装清单:
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产品编号
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产品名称
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包装
|
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XY1606
|
Cyclin B1
Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
Cyclin B1 Rabbit
Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10