Phospho-GSK-3β (Ser 9) Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1531
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Phospho-GSK-3β (Ser 9) Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IF, IHC,
ICC
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H
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47.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:2,000
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-
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1:50-1:200
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1:50-1:200
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1:50-1:200
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-
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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Phospho-GSK-3β (Ser9) Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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2932(Human)
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SwissProt
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P49841(Human)
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Synonyms
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Glycogen
Synthase Kinase 3 Beta
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Category
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PI3K/Akt/mTOR
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Background
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Glycogen
synthase kinase-3α (GSK-3α) and GSK-3β are highly similar isoforms of serine/ threonine kinases that
regulate metabolic enzymes and transcription factors, which are responsible
for coordinating processes such as glycogen synthesis and cell adhesion.
GSK-3β activity is also required for nuclear activity
of Rel dimers, which mediate an anti-apoptotic response to TNFα in mice. GSK-3 catalytic kinase activity is controlled through
differential phosphorylation of serine/threonine residues, which have an
inhibitory effect, and tyrosine residues, which have an activating effect.
Growth factor stimulation of mammalian cells expressing GSK-3α and GSK-3β induces phosphorylation of Ser
21 and Ser 9, respectively, through a phosphatidylinositol 3-kinase (PI 3-K)-protein
kinase B (PKB)-dependent pathway, thereby enhancing proliferative signals.
Additionally, GSK-3 physically associates with cAMP-dependent protein kinase
A (PKA), which phosphorylates Ser 21 of GSK-3α or Ser
9 of GSK-3β and inactivates both forms. GSK-3α/β is positively regulated by
phosphorylation on Tyr 279 and Tyr 216, respectively. Activated GSK-3α/β participates in energy metabolism,
neuronal cell development, and body pattern formation. Tyrosine
dephosphorylation of GSK-3 is involved in its extracellular signal-dependent
inactivation.
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包装清单:
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产品编号
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产品名称
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包装
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XY1531
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Phospho-GSK-3β (Ser9) Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
Phospho-GSK-3β (Ser9) Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10