CDC7 Kinase Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1459
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CDC7 Kinase
Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, FC
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H, M, R
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64.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:5,000
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-
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-
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-
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-
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1:50-1:100
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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CDC7 Kinase
Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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8317(Human);
12545(Mouse); 360908(Rat)
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SwissProt
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O00311(Human);
Q9Z0H0(Mouse); D4A8S5(Rat)
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Synonyms
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CDC7L1;
HsCDC7; Hsk1; huCDC7
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Category
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Cell Cycle
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Background
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The Dbf4/Cdc7
protein kinase is essential for the activation of replication origins during
S phase. Cdc7/Dbf4 efficiently phosphorylates several proteins that are
required for the initiation of DNA replication, including five of the six
minichromosome maintenance (Mcm) proteins and the p180 subunit of DNA
polymerase a-primase. This protein complex consists of the catalytic subunit
Cdc7 associating with the regulatory and activating subunit Dbf4. The kinase
activity of the complex is regulated throughout the cell cycle, mainly by
fluctuating levels of Dbf4. Cdc7 is consistently expressed throughout the
cell cycle, while the expression of Dbf4 is absent during G1 phase and
accumulates during S and G2 phases. The anaphase-promoting complex rapidly
degrades Dbf4 at the time of chromosome segregation, and the stability of
Dbf4 remains low during pre-Start G1 phase. The coordinated degradation of
Dbf4 and the time of chromosome separation is important to ensuring that
prereplicative complexes, which assemble XYter chromosome segregation, do not
immediately refire.
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包装清单:
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产品编号
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产品名称
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包装
|
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XY1459
|
CDC7 Kinase
Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
CDC7 Kinase
Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10