Phospho-EGFR (Tyr1092) Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1429
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Phospho-EGFR
(Tyr1092) Rabbit Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IF, IHC,
ICC
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H, M
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170.0kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:2,000
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-
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1:50-1:200
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1:50-1:200
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1:50-1:200
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-
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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Phospho-EGFR
(Tyr1092) Rabbit Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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1956(Human);
13649(Mouse)
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SwissProt
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P00533(Human);
Q01279(Mouse)
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Synonyms
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ERBB; ERBB1;
HER1; NISBD2; PIG61; mENA
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Category
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Protein
Tyrosine Kinase
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Background
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Epidermal
growth factor mediates its effects on cell growth through its inter-action
with a cell surface glycoprotein designated the EGF receptor. Binding of EGF
or TGF alpha to the EGF receptor activates tyrosine-specific protein kinase
activity intrinsic to the EGF receptor. The carboxy terminal tyrosine
residues on EGFR, Tyr 1068 and Tyr 1173, are the major sites of
autophosphorylation, which occurs as a result of EGF binding. Once activated,
EGFR mediates the binding of the phosphotyrosine binding (PTB) domain of GRB2
through direct interactions with Tyr 1068 and Tyr 1086 and through indirect
interactions with Tyr 1173 in the Ras signaling pathway. Tyr 1173 of EGFR
also functions as a kinase substrate. Phosphorylation of Tyr 992, Tyr 1068
and Tyr 1086 is required for conformational change in the C-terminal tail of
the EGF receptor.
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包装清单:
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产品编号
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产品名称
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包装
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XY1429
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Phospho-EGFR
(Tyr1092) Rabbit Monoclonal Antibody
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50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
Phospho-EGFR
(Tyr1092) Rabbit Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10