EGFR Rabbit Monoclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY1330
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EGFR Rabbit
Monoclonal Antibody
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50μl
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618.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IP, IF,
IHC, ICC, FC
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H, M, R
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150/50 kDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1,000-1:2,000
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1:10-1:50
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1:100-1:500
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1:50-1:200
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1:100-1:500
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1:50-1:100
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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EGFR Rabbit
Monoclonal Antibody
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Category
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Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
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Isotype
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IgG
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Purification
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XYfinity
purification
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About the
Immunogen
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Immunogen
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Recombinant
protein
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Gene ID
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1956(Human);
13649(Mouse)
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SwissProt
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P00533(Human);
Q01279(Mouse); Q9WTS1(Rat)
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Synonyms
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ERBB; ERBB1;
HER1; NISBD2; PIG61; mENA
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Category
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Protein
Tyrosine Kinase
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Background
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The EGF
receptor family comprises several related receptor tyrosine kinases that are
frequently overexpressed in a variety of carcinomas. Members of this receptor
family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4
(HER4), which form either homodimers or heterodimers upon ligand binding.
Exons in the EGFR gene product are frequently either deleted or duplicated to
produce deletion mutants (DM) or tandem duplication mutants (TDM),
respectively, which are detected at various molecular weights. EGFR binds
several ligands, including epidermal growth factor (EGF), transforming growth
factor α (TGFα), Amphiregulin and
heparin binding-EGF (HB-EGF). Ligand binding promotes the internalization of
EGFR via Clathrin-coated pits and its subsequent degradation in response to
its intrinsic tyrosine kinase. EGFR is involved in organ morphogenesis and
maintenance and repair of tissues, but upregulation of EGFR is associated
with tumor progression. The oncogenic effects of EGFR include initiation of DNA
synthesis, enhanced cell growth, invasion and metastasis. Abrogation of EGFR
results in cell cycle arrest, apoptosis or dedifferentiation of cancer cells,
suggesting that EGFR may be an effective therapeutic target.
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包装清单:
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产品编号
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产品名称
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包装
|
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XY1330
|
EGFR Rabbit
Monoclonal Antibody
|
50μl
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XY050
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Western一抗稀释液
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50ml
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-
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说明书
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1份
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保存条件:
EGFR Rabbit
Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10