MSH6 Rabbit Monoclonal Antibody
|
产品编号
|
产品名称
|
产品包装
|
产品价格
|
|
XY1321
|
MSH6 Rabbit
Monoclonal Antibody
|
50μl
|
618.00元
|
|
来源
|
用途
|
交叉反应性
|
分子量
|
|
Rabbit
|
WB, IF, IHC,
ICC
|
H, M, R
|
153.0kDa
|
WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
|
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
|
1:1,000-1:5,000
|
-
|
1:50-1:200
|
1:50-1:200
|
1:50-1:200
|
-
|
-
|
-
|
抗体详细信息如下:
|
About this
Antibody
|
|
Name
|
MSH6 Rabbit
Monoclonal Antibody
|
|
Category
|
Rabbit
Monoclonal Antibody (RabMAb); Primary antibody
|
|
Isotype
|
IgG
|
|
Purification
|
XYfinity
purification
|
|
About the
Immunogen
|
|
Immunogen
|
Recombinant
protein
|
|
Gene ID
|
2956(Human);
17688(Mouse); 100360342(Rat)
|
|
SwissProt
|
P52701(Human);
P54276(Mouse)
|
|
Synonyms
|
-
|
|
Category
|
Epigenetics
|
|
Background
|
Multiple
pathways promote short-sequence recombination (SSR) in Saccharomyces
cerevisiae. When gene conversion is initiated by a double-strand break (DSB),
any nonhomologous DNA that may be present at the ends must be removed before
new DNA synthesis can be initiated. Removal of a 3' nonhomologous tail in S.
cerevisiae depends on the nucleotide excision repair endonuclease Rad1/Rad10
and also on the mismatch repair proteins Msh2 and Msh3. Msh2 and Msh3, which
function in mitotic recombination, recognize not only heteroduplex loops and
mismatched basepairs, but also branched DNA structures with a free 3' tail.
Msh2 and Msh6 form a protein complex required to repair mismatches generated
during DNA replication. Yeast Msh2-Msh6 interact asymmetrically with the DNA
through base-specific stacking and hydrogen bonding interactions and backbone
contacts. The importance of these contacts decreases with increasing distance
from the mismatch, implying that interactions at or near the mismatch are
important for binding in a kinked DNA conformation.
|
包装清单:
|
产品编号
|
产品名称
|
包装
|
|
XY1321
|
MSH6 Rabbit
Monoclonal Antibody
|
50μl
|
|
XY050
|
Western一抗稀释液
|
50ml
|
|
-
|
说明书
|
1份
|
保存条件:
MSH6 Rabbit
Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项:
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10