HSP70 Rabbit Polyclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY0189
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HSP70 Rabbit
Polyclonal Antibody
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50μl
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416.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IHC, ICC,
IF
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H, M, R
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70KDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:500-1:1000
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-
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1:200
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1:200
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1:200
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-
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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HSP70 Rabbit
Polyclonal Antibody
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Category
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Polyclonal
antibody(pAb); Primary antibody
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Isotype
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IgG
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Purification
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Peptide XYfinity
purified
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About the
Immunogen
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Immunogen
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This antibody
is produced by immunizing rabbits with a synthetic peptide (KLH-coupled)
corresponding to HSP70.
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Gene ID
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3304
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SwissProt
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P08107
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Synonyms
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HSP72; HSPA1;
HSP70I; HSP70-1; HSP70-1A;HSPA1A
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Category
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Protein
Folding and TrXYficking
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Background
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The 70
kilodalton heat shock proteins (Hsp70s) are a family of conserved
ubiquitously expressedheat shock proteins. Proteins with similar structure
exist in virtually all living organisms. The Hsp70s are an important part of
the cell's machinery for protein folding, and help to protect cells from
stress. When not interacting with a substrate peptide, Hsp70 is usually in an
ATP bound state. Hsp70 by itself is characterized by a very weak ATPase
activity, such that spontaneous hydrolysis will not occur for many minutes.
As newly synthesized proteins emerge from the ribosomes, the substrate
binding domain of Hsp70 recognizes sequences of hydrophobic amino acid
residues, and interacts with them. This spontaneous interaction is
reversible, and in the ATP bound state Hsp70 may relatively freely bind and
release peptides. However, the presence of a peptide in the binding domain
stimulates the ATPase activity of Hsp70, increasing its normally slow rate of
ATP hydrolysis.
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包装清单：
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产品编号
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产品名称
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包装
|
|
XY0189
|
HSP70 Rabbit
Polyclonal Antibody
|
50μl
|
|
AZ050
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Western一抗稀释液
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50ml
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|
—
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说明书
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1份
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保存条件：
HSP70 Rabbit
Polyclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10