Glutathione Peroxidase 1 Mouse Monoclonal Antibody
|
产品编号
|
产品名称
|
产品包装
|
产品价格
|
|
XY0162
|
Glutathione
Peroxidase 1 Mouse Monoclonal Antibody
|
50μl
|
416.00元
|
|
来源
|
用途
|
交叉反应性
|
分子量
|
|
Mouse
|
WB, FC, IHC,
ICC, IF
|
H
|
22KDa
|
WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin
Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
|
WB
|
IP
|
IF
|
IHC
|
ICC
|
FC
|
ELISA
|
ChIP
|
|
1:1000
|
-
|
1:200
|
1:200
|
1:200
|
1:100-1:200
|
-
|
-
|
抗体详细信息如下:
|
About this
Antibody
|
|
Name
|
Glutathione
Peroxidase 1 Mouse Monoclonal Antibody
|
|
Category
|
Monoclonal
antibody(mAb); Primary antibody
|
|
Isotype
|
IgG2b
|
|
Purification
|
ProA XYfinity
purified
|
|
About the
Immunogen
|
|
Immunogen
|
This antibody
is produced by immunizing mice with a recombinant protein corresponding to a
region of GPX1.
|
|
Gene ID
|
2876
|
|
SwissProt
|
P07203
|
|
Synonyms
|
GPX1; GSHPX1;
MGC14399; MGC88245
|
|
Category
|
Metabolism
|
|
Background
|
GPX1 is
ubiquitously expressed in many tissues, where it protects cells from
oxidative stress. Within cells, it localizes to the cytoplasm and
mitochondria. As a glutathione peroxidase, GPx1 functions in the
detoxification of hydrogen peroxide, specifically by catalyzing the reduction
of hydrogen peroxide to water. The glutathione peroxidase also catalyzes the
reduction of other organic hydroperoxides, such as lipid peroxides, to the
corresponding alcohols. GPx1 typically uses glutathione (GSH) as the reductant,
but when glutathione synthetase (GSS) is, as in brain mitochondria, γ-glutamylcysteine can serve as the reductant instead. The protein
encoded by this gene protects from CD95-induced apoptosis in cultured breast
cancer cells and inhibits 5-lipoxygenase in blood cells, and its
overexpression delays endothelial cell death and increases resistance to
toxic challenges, especially oxidative stress. This protein is one of only a
few proteins known in higher vertebrates to contain selenocysteine, which occurs
at the active site of glutathione peroxidase and is coded by the nonsense
(stop) codon TGA.
|
包装清单：
|
产品编号
|
产品名称
|
包装
|
|
XY0162
|
Glutathione
Peroxidase 1 Mouse Monoclonal Antibody
|
50μl
|
|
AZ050
|
Western一抗稀释液
|
50ml
|
|
—
|
说明书
|
1份
|
保存条件：
Glutathione
Peroxidase 1 Mouse Monoclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物。
注意事项：
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10