β-Catenin Rabbit Polyclonal Antibody
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产品编号
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产品名称
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产品包装
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产品价格
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XY0066
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β-Catenin Rabbit Polyclonal Antibody
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50μl
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416.00元
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来源
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用途
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交叉反应性
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分子量
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Rabbit
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WB, IHC, FC
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H, M, R
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85KDa
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WB, Western blot;
IP, Immunoprecipitation; IF, Immunofluorescence; IHC, Immunohistochemistry;
ICC,
Immunocytochemistry; FC, Flow Cytometry; ELISA, Enzyme-linked Immunosorbent
Assay; ChIP, Chromatin Immunoprecipitation Assay.
H, Human; M,
Mouse; R, Rat; C, Chicken; Cw, Cow; Dg, Dog; Gp, Guinea pig; Hm, Hamster; Hr,
Horse; Mk,
Monkey; Pg, Pig; Rb, Rabbit; S, Sheep; Z, ZebrXYish; All, all species expected.
配套提供了Western一抗稀释液，可以用于Western检测或其它适当用途时的一抗稀释。
建议抗体使用时的稀释比例如下(实际使用时需根据抗原水平的高低作适当调整)：
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WB
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IP
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IF
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IHC
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ICC
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FC
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ELISA
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ChIP
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1:1000
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-
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-
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1:200
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-
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1:50-1:100
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-
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-
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抗体详细信息如下:
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About this
Antibody
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Name
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β-Catenin Rabbit Polyclonal Antibody
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Category
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Polyclonal
antibody(pAb); Primary antibody
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Isotype
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IgG
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Purification
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Peptide XYfinity
purified
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About the
Immunogen
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Immunogen
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This antibody
is produced by immunizing rabbits with a synthetic peptide (KLH-coupled)
corresponding to β-catenin.
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Gene ID
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1499
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SwissProt
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P35222
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Synonyms
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CTNNB;
DKFZp686D02253; FLJ25606; FLJ37923; β-catenin;
Beta-Catenin
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Category
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Developmental
Biology
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Background
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β-Catenin is a key downstream effector in the Wnt signaling
pathway. It is implicated in two major biological processes in vertebrates:
early embryonic development and tumorigenesis. Phosphorylation at Ser-552 by
AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated
transcription. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances
TBP binding. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41.
Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell
lines. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and
accumulates in the nucleus, where it acts as a coactivator for transcription
factors of the TCF/LEF family, leading to activate Wnt responsive genes.
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包装清单：
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产品编号
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产品名称
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包装
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XY0066
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β-Catenin Rabbit Polyclonal Antibody
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50μl
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AZ050
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Western一抗稀释液
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50ml
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—
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说明书
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1份
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保存条件：
β-Catenin
Rabbit Polyclonal Antibody -20℃保存，Western一抗稀释液-20℃或4℃保存，一年有效。Western一抗稀释液优先推荐4℃保存，长期不使用可以考虑-20℃保存，但冻融可能会导致出现轻微的浑浊和少量不溶物
注意事项：
如果本抗体用于Western blot (WB)、**荧光(IF)、**细胞化学(ICC)等实验，请注意回收使用过的稀释抗体?；厥盏目固逋ǔＶ辽倏梢灾馗词褂?span>5-10