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产品名称: Recombinant Rat MIF
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Rat MIF由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Rat MIF所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Rat MIF
的详细介绍
Recombinant Rat MIF
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产品编号
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产品名称
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产品包装
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产品价格
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XY7365-1mg
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Recombinant
Rat MIF
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1mg
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12354.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Rat
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81683
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P30904
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E. coli
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115aa
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12.5kDa
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－
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About this
Antibody
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Name
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Recombinant
Rat MIF (Recombinant Rat Migration Inhibitor Factor; rRtMIF)；重组大鼠移动抑制因子
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Synonyms
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EC 5.3.2.1; EC
5.3.3.12; GIFmacrophage migration inhibitory factor; GLIF;
Glycosylation-inhibiting factor; L-dopachrome isomerase; L-dopachrome
tautomerase; macrophage migration inhibitory factor (glycosylation-inhibiting
factor); MMIF; Phenylpyruvate tautomerase
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Purity
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>97% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Test in
process.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in 20mM Tris, pH8.0,
150mM NaCl, 3% trehalose.
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Endotoxin
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Less than 1EU/μg of rRtMIF as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Others
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Background
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Macrophage
migration inhibitory factor (MIF ormMIF), also named as
glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, or
phenylpyruvate tautomerase, is a protein encoded by the MIF gene. It is
released from white blood cells by bacterial antigen stimulation to trigger
an acute immune response, or by glucocorticoids to counter-act the inhibitory
effects of glucocorticoids on immune system. MIF is a homotrimer of which
each subunit contains 115 amino acids. As mentioned above, MIF is involved in
the innate immune response to bacterial pathogens and counter-acts the
anti-inflammatory activity of glucocorticoids. Furthermore, it also plays a
role as mediator in regulating the function of macrophages in host defense
and has phenylpyruvate tautomerase and dopachrome tautomerase activity in
vitro. Rat MIF is 99%, 90%, 89%, and 89%a.a. identical to human, murine,
porcine and bovine, respectively.
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Amino Acid
Sequence
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MPMFIVNTNV PRASVPEGFL SELTQQLAQA
TGKPAQYIAV HVVPDQLMTF SGTSDPCALC SLHSIGKIGG AQNRNYSKLL CGLLSDRLHI SPDRVYINYY
DMNAANVGWN GSTFA
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包装清单:
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产品编号
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产品名称
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包装
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XY7365-10μg
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Recombinant
Rat MIF
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10μg
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XY7365-50μg
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Recombinant
Rat MIF
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50μg
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XY7365-100μg
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Recombinant
Rat MIF
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100μg
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XY7365-1mg
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Recombinant
Rat MIF
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。