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产品名称: Recombinant Human ApoA-I
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Human ApoA-I由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。Recombinant Human ApoA-I所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Human ApoA-I
的详细介绍
Recombinant Human ApoA-I
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产品编号
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产品名称
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产品包装
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产品价格
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XY7280-1mg
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Recombinant
Human ApoA-I
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1mg
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5665.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Human
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335
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P02647
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E. coli
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243aa
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28.1kDa
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-
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About this
protein
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Name
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Recombinant
Human ApoA-I (Recombinant Human Apolipoprotein A-I; rHuApoA-I);重组人载脂蛋白A-I
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Synonyms
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Alp-1; APOA1;
Brp-14; Ltw-1; Lvtw-1; Sep-1; Sep-2; APOA1; Apo-AI; apo-AI; ApoA-I; apoA-I;
Apolipoprotein A1; apolipoprotein A-I; MGC117399
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Purity
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>98% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Data Not
Available.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μM filtered concentrated solution in PBS, pH7.4.
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Endotoxin
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Less than
0.1EU/μg of rHuApoA-I as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing
0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should be
apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Others
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Background
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Apolipoprotein
A-I encoded by the APOA1 gene is the major protein component of high density
lipoprotein (HDL) in plasma. It has a specific role in lipid metabolism.
Apolipoprotein A-I participates in the reverse transport of cholesterol from
tissues to the liver for excretion by promoting cholesterol efflux from
tissues and by acting as a cofactor for the lecithin cholesterol
acyltransferase (LCAT). Apolipoprotein A-I is also isolated as a prostacyclin
(PGI2) stabilizing factor and thus may have an anticlotting effect.
Apolipoprotein A-I has been shown to interact with ABCA1, GPLD1 and PLTP.
Apolipoprotein A-I binds to lipopolysaccharide or endotoxin and has a major
role in the anti-endotoxin function of HDL. Mature human ApoA-1 shares 65%
and 62%a.a. sequence identity with murine and rat ApoA-1, respectively. It
has an approximate molecular weight of 28.1kDa.
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Amino Acid
Sequence
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DEPPQSPWDR VKDLATVYVD VLKDSGRDYV
SQFEGSALGK QLNLKLLDNW DSVTSTFSKL REQLGPVTQE FWDNLEKETE GLRQEMSKDL EEVKAKVQPY
LDDFQKKWQE EMELYRQKVE PLRAELQEGA RQKLHELQEK LSPLGEEMRD RARAHVDALR THLAPYSDEL
RQRLAARLEA LKENGGARLA EYHAKATEHL STLSEKAKPA LEDLRQGLLP VLESFKVSFL SALEEYTKKL
NTQ
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包装清单:
|
产品编号
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产品名称
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包装
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|
XY7280-20μg
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Recombinant
Human ApoA-I
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20μg
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XY7280-100μg
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Recombinant
Human ApoA-I
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100μg
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XY7280-1mg
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Recombinant
Human ApoA-I
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用,不得用于临床诊断或**,不得用于食品或药品,不得存放于普通住宅内。
为了您的**和健康,请穿实验服并戴一次性手套操作。