Recombinant Human DHH C23II
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产品名称: Recombinant Human DHH C23II
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Human DHH C23II由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Human DHH C23II所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Human DHH C23II
的详细介绍
Recombinant Human DHH C23II
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产品编号
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产品名称
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产品包装
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产品价格
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XY7239-1mg
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Recombinant
Human DHH C23II
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1mg
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14367.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Human
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50846
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O43323
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E. coli
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177aa
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19.9kDa
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－
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About this
Antibody
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Name
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Recombinant
Human DHH C23II (Recombinant Human Desert Hedgehog Cys23IleIle; rHuDHH C23II)；重组人沙漠刺猬因子C23II
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Synonyms
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DHH; desert
hedgehog; desert hedgehog (Drosophila) homolog; desert hedgehog homolog;
desert hedgehog protein; HHG-3MGC35145
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Purity
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>96% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The ED50 as determined by its
ability to induce alkaline phosphatase production by C3H10T1/2(CCL-226) cells
is 15-45μg/ml.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in 10mM PB, pH6.0,
300mM NaCl.
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Endotoxin
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Less than 1EU/μg of rHuDHH C23II as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Others
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Background
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Desert
hedgehog protein (DHH), also named HHG-3, is belonging to the hedgehog (Hh)
family that are highly conserved proteins which are widely represented
throughout the animal kingdom. This protein is predicted to be made as a
precursor that is autocatalytically cleaved and the biologically active form
of Hh molecules corresponds to approximately the N-terminal one half of the
precursor molecule. The N-terminal portion is soluble and contains the
signaling activity while the C-terminal portion is involved in processing.
More importantly, the C-terminal product covalently attaches a cholesterol
moiety to the N-terminal product, restricting the N-terminal product to the
cell surface and preventing it from freely diffusing throughout the organism.
DHH is essential for a variety of patterning events during development as
intercellular signal. It may function as a spermatocyte survival factor in
the testes and play an important role in testes development. Mature human DHH
shares 97% amino acid sequence identity with murine DHH.
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Amino Acid
Sequence
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IIGPGRGPVG RRRYARKQLV PLLYKQFVPG
VPERTLGASG PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE RCKERVNALA IAVMNMWPGV
RLRVTEGWDE DGHHAQDSLH YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH VHVSVKADNS
LAVRAGG
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包装清单:
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产品编号
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产品名称
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包装
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XY7239-5μg
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Recombinant
Human DHH C23II
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5μg
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XY7239-25μg
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Recombinant
Human DHH C23II
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25μg
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XY7239-100μg
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Recombinant
Human DHH C23II
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100μg
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XY7239-1mg
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Recombinant
Human DHH C23II
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。