Species

Gene ID

Accession

Source

Length

MW

Tag

Human

11065

O00762

E. coli

191aa

21.1kDa

His

About this Antibody

Name

Recombinant Human UBE2C, His-tag (Recombinant Human Ubiquitin Conjugating Enzyme E2 C, His-tag; rHuUBE2C, His-tag)；重组人泛素结合酶E2C，His标签

Synonyms

UBE2C; cyclin-selective ubiquitin carrier protein; dJ447F3.2; EC 6.3.2.19; UbcH10; UBCH10mitotic-specific ubiquitin-conjugating enzyme; Ubiquitin carrier protein C; ubiquitin carrier protein E2-C; ubiquitin-conjugating enzyme E2 C; ubiquitin-conjugating enzyme E2C; Ubiquitin-protein ligase C

Purity

>95% by SDS-PAGE and HPLC analyses.

Biological Activity

Data is not available.

Physical Appearance

Sterile Colorless liquid.

Formulation

A 0.2μm filtered concentrated solution in 50mM HEPES, pH8.0, with 125mM NaCl, 10% Glycerol, 5% Trehalose, 1mM DTT.

Endotoxin

Less than 1EU/μg of rHuUBE2C, His-tag as determined by LAL method.

Reconstitution

N/A

Category

Ubiquitins

Background

Ubiquitin Conjugating Enzyme E2 C belongs to the ubiquitin-conjugating enzyme family and is encoded by the UBE2C gene in humans. The ubiquitin-conjugating enzymes, also known as E2 enzymes and more rarely as ubiquitin-carrier enzymes, take part in the second step in the ubiquitination reaction. In this reaction, E1 activates the ubiquitin by covalently attaching the molecule to its active site cysteine residue. The activated ubiquitin is then transferred to an E2 cysteine and then the E2 molecule binds E3 via a structurally conserved binding region. The UBE2C catalyzes the destruction of cyclins A and B in conjunction with the anaphase-promoting complex, and therefore, plays an important role in the control of the cell exit from mitosis. This activity is essential at the end of mitosis for the inactivation of their partner kinase Cdc2 and exit from mitosis into G1 of the next cell cycle. In addition, UBCH10 bears homology to yeast PAS2, a gene that is essential for biogenesis of peroxisomes. UBCH10 is useful for in vitro ubiquitinylation reactions.

Amino Acid Sequence

MHHHHHHAMG IRMASQNRDP AATSVAAARK GAEPSGGAAR GPVGKRLQQE LMTLMMSGDK GISAFPESDN LFKWVGTIHG AAGTVYEDLR YKLSLEFPSG YPYNAPTVKF LTPCYHPNVD TQGNICLDIL KEKWSALYDV RTILLSIQSL LGEPNIDSPL NTHAAELWKN PTAFKKYLQE TYSKQVTSQE P

产品编号

产品名称

包装

XY7118-10μg

Recombinant Human UBE2C, His-tag

10μg

XY7118-50μg

Recombinant Human UBE2C, His-tag

50μg

XY7118-100μg

Recombinant Human UBE2C, His-tag

100μg

XY7118-1mg

Recombinant Human UBE2C, His-tag

1mg

-

说明书

1份