Recombinant Human ME2, His-tag
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产品名称: Recombinant Human ME2, His-tag
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Human ME2, His-tag由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Human ME2, His-tag所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Human ME2, His-tag
的详细介绍
Recombinant Human ME2, His-tag
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产品编号
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产品名称
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产品包装
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产品价格
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XY7014-1mg
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Recombinant
Human ME2, His-tag
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1mg
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14568.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Human
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4200
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P23368
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E. coli
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573aa
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64.4kDa
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His
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About this
Antibody
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Name
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Recombinant
Human ME2, His-tag (Recombinant Human Malic Enzyme 2, His-tag; rHuME2,
His-tag)；重组人苹果酸酶2，His标签
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Synonyms
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NAD-dependent
malic enzyme, mitochondrial; NAD-ME; Malic enzyme 2; ME2
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Purity
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>95% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Malic Enzyme
activity was assayed spectrophotometrically at 340nm as described in Mandela
and Sauer (1975). The standard reaction mixture contained 50mM Tris-HCl, 3mM
MnCl2, 5mM malate, 0.12mM NADP+, 2.5mM fumarate. Assay
was performed in a Beckman spectrophotometer. The Km value is 1.5±0.6mM.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in PBS, pH7.4.
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Endotoxin
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Less than 1EU/μg of rHuME2, His-tag as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Enzymes
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Background
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NAD-dependent
malic enzyme (ME2), mitochondrial is a protein that in humans is encoded by
the ME2 gene. This gene encodes a mitochondrial NAD-dependent malic enzyme, a
homotetrameric protein, which catalyzes the oxidative decarboxylation of
malate to pyruvate. Three different isoforms of ME are known to be in mammalian
tissues: a strictly cytosolic NADP+-dependent enzyme, an NADP+-dependent
mitochondriail isoform, and a mitochondrial isoenzyme that is able to use
both NAD+ and NADP+ but is more effective with NAD+.
The mammalian isoforms size is about 62-64kDa. A native size of 240,000 Da
proposes a tetrameric structure for the active enzyme.
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Amino Acid
Sequence
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MLHIKEKGKP LMLNPRTNKG MAFTLQERQM
LGLQGLLPPK IETQDIQALR FHRNLKKMTS PLEKYIYIMG IQERNEKLFY RILQDDIESL MPIVYTPTVG
LACSQYGHIF RRPKGLFISI SDRGHVRSIV DNWPENHVKA VVVTDGERIL GLGDLGVYGM GIPVGKLCLY
TACAGIRPDR CLPVCIDVGT DNIALLKDPF YMGLYQKRDR TQQYDDLIDE FMKAITDRYG RNTLIQFEDF
GNHNAFRFLR KYREKYCTFN DDIQGTAAVA LAGLLAAQKV ISKPISEHKI LFLGAGEAAL GIANLIVMSM
VENGLSEQEA QKKIWMFDKY GLLVKGRKAK IDSYQEPFTH SAPESIPDTF EDAVNILKPS TIIGVAGAGR
LFTPDVIRAM ASINERPVIF ALSNPTAQAE CTAEEAYTLT EGRCLFASGS PFGPVKLTDG RVFTPGQGNN
VYIFPGVALA VILCNTRHIS DSVFLEAAKA LTSQLTDEEL AQGRLYPPLA NIQEVSINIA IKVTEYLYAN
KMAFRYPEPE DKAKYVKERT WRSEYDSLLP DVYEWPESAS SPPVITEHHH HHH
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包装清单:
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产品编号
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产品名称
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包装
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XY7014-5μg
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Recombinant
Human ME2, His-tag
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5μg
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XY7014-25μg
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Recombinant
Human ME2, His-tag
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25μg
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XY7014-100μg
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Recombinant
Human ME2, His-tag
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100μg
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XY7014-1mg
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Recombinant
Human ME2, His-tag
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。