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产品名称: Recombinant Rat FGF-21
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Rat FGF-21由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Rat FGF-21所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Rat FGF-21
的详细介绍
Recombinant Rat FGF-21
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产品编号
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产品名称
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产品包装
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产品价格
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XY6366-1mg
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Recombinant
Rat FGF-21
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1mg
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15611.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Rat
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170580
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Q8VI80
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E. coli
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180aa
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19.7kDa
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－
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About this
Antibody
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Name
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Recombinant
Rat FGF-21 (Recombinant Rat Fibroblast Growth Factor-21; rRtFGF-21)；重组大鼠成纤维细胞生长因子21
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Synonyms
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FGF-21;
fibroblast growth factor 21
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Purity
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>95% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The ED50 as determined by
thymidine uptake assay using FGF-receptors transfected BaF3 cells is less
than 0.5μg/ml, corresponding to a specific activity of >2.0×103IU/mg in the presence of 5μg/ml
of rMuKlotho-β and 10μg/ml of
heparin.
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Physical
Appearance
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White
lyophilized powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in PBS, 500mM NaCl,
pH7.4.
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Endotoxin
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Less than 1EU/μg of rRtFGF-21 as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should
be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Cytokine
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Background
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Fibroblast
growth factor 21 (FGF-21) encoded by the FGF-21 gene belongs to the large FGF
family and it is specifically induced by HMGCS2 activity. In mice, brown
adipose tissue becomes a source of systemic FGF-21 after cold exposure.
FGF-21 stimulates glucose uptake in differentiated adipocytes via the
induction of glucose transporter SLC2A1/GLUT1 expression (but not
SLC2A4/GLUT4 expression) and the activity depends on the presence of KLB.
Recombinant rat FGF-21 contains 180 amino acids residues and show limited
binding to heparin. In addition, rat FGF-21 respectively shows 81% and
92%a.a. identity to human and murine FGF-21, and it show activity on murine
and human cells.
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Amino Acid
Sequence
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AYPISDSSPL LQFGGQVRQR YLYTDDDQDT
EAHLEIREDG TVVGTAHRSP ESLLELKALK PGVIQILGVK ASRFLCQQPD GTLYGSPHFD PEACSFRELL
LKDGYNVYQS EAHGLPLRLP QKDSQDPATR GPVRFLPMPG LPHEPQEQPG VLPPEPPDVG SSDPLSMVEP
LQGRSPSYAS
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包装清单:
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产品编号
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产品名称
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包装
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|
XY6366-5μg
|
Recombinant
Rat FGF-21
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5μg
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XY6366-25μg
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Recombinant
Rat FGF-21
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25μg
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XY6366-100μg
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Recombinant
Rat FGF-21
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100μg
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XY6366-1mg
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Recombinant
Rat FGF-21
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。