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产品名称: Recombinant Rat SCF
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Rat SCF由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Rat SCF所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Rat SCF
的详细介绍
Recombinant Rat SCF
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产品编号
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产品名称
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产品包装
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产品价格
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XY6321-1mg
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Recombinant
Rat SCF
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1mg
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15444.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Rat
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60427
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P21581
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E. coli
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165aa
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18.5kDa
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－
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About this
Antibody
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Name
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Recombinant
Rat SCF (Recombinant Rat Stem Cell Factor; rRtSCF)；重组大鼠干细胞生长因子
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Synonyms
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c-kit Ligand;
KITLG; MGF; SLF; DKFZXY686F2250; familial progressive hyperpigmentation 2;
FPH2; KIT ligand; Kitl; KL-1; Mast cell growth factor; MGFSHEP7; SCFStem cell
factor; SFc-Kit ligand; steel factor
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Purity
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>95% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The ED50 as determined by a
cell proliferation assay using murine MC/9-2 mast cells is less than 5ng/ml,
corresponding to a specific activity of >2.0×105IU/mg.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in PBS, pH7.4.
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Endotoxin
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Less than 1EU/μg of rRtSCF as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Cytokine
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Background
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Stem Cell
Factor (SCF) which binds to the c-Kit receptor is produced by fibroblasts and
endothelial cells. The soluble and transmembrane forms of the protein are
formed by alternative splicing of the same RNA transcript and the presence of
both soluble and transmembrane SCF is required for normal hematopoietic
function. SCF plays an important role in hematopoiesis, spermatogenesis, and
melanogenesis. In addition, it also promotes mast cell adhesion, migration,
proliferation, and survival 3. Rat SCF shares 75%-90%a.a. sequence identity
with canine, feline, mouse, and human SCF. Furthermore, rat SCF is active on
mouse and human cells, but human SCF is only weakly active on mouse cells.
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Amino Acid
Sequence
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MQEICRNPVT DNVKDITKLV ANLPNDYMIT
LNYVAGMDVL PSHCWLRDMV THLSVSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVAC MEENAPKNVK
ESLKKPETRN FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA
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包装清单:
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产品编号
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产品名称
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包装
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XY6321-2μg
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Recombinant
Rat SCF
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2μg
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XY6321-10μg
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Recombinant
Rat SCF
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10μg
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XY6321-100μg
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Recombinant
Rat SCF
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100μg
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XY6321-1mg
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Recombinant
Rat SCF
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。