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产品名称: Recombinant Murine CNTF
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Murine CNTF由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。Recombinant Murine CNTF所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Murine CNTF
的详细介绍
Recombinant Murine CNTF
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产品编号
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产品名称
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产品包装
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产品价格
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XY6155-1mg
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Recombinant
Murine CNTF
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1mg
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15611.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Mouse
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12803
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P51642
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E. coli
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197aa
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22.5kDa
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-
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About this
Antibody
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Name
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Recombinant
Murine CNTF (Recombinant Murine Ciliary Neurotrophic Factor; rMuCNTF);Recombinant Mouse CNTF ; 重组小鼠人睫状神经营养因子
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Synonyms
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ciliary
neurotrophic factor; HCNTF
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Purity
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>95% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The ED50 as determined by a
cell proliferation assay using human TF-1 cells is less than 30ng/ml,
corresponding to a specific activity of >3.3×104IU/mg.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in 2X PBS, pH7.4, 2%
trehalose.
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Endotoxin
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Less than 1EU/μg of rMuCNTF as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Cytokine
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Background
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Ciliary
neurotrophic factor (CNTF) is a polypeptide hormone whose actions appear to
be restricted to the nervous system where it promotes neurotransmitter
synthesis and neurite outgrowth in certain neuronal populations. CNTF was
initially identified as a trophic factor for embryonic chick ciliary
parasympathetic neurons in culture. Furthermore, the protein is also a potent
survival factor for neurons and oligodendrocytes and may be relevant in
reducing tissue destruction during inflammatory attacks. In addition, CNTF is
useful for treatment of motor neuron disease and it could reduce food intake
without causing hunger or stress. Recombinant murine CNTF containing 197
amino acids and it shares 82% and 95%a.a. sequence identity with human and
rat CNTF.
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Amino Acid
Sequence
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AFAEQSPLTL HRRDLCSRSI WLARKIRSDL
TALMESYVKH QGLNKNISLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT
EGDFHQAIHT LTLQVSAFAY QLEELMALLE QKVPEKEADG MPVTIGDGGL FEKKLWGLKV LQELSQWTVR
SIHDLRVISS HHMGISAHES HYGAKQM
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包装清单:
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产品编号
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产品名称
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包装
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XY6155-5μg
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Recombinant
Murine CNTF
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5μg
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XY6155-25μg
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Recombinant
Murine CNTF
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25μg
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XY6155-100μg
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Recombinant
Murine CNTF
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100μg
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XY6155-1mg
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Recombinant
Murine CNTF
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用,不得用于临床诊断或**,不得用于食品或药品,不得存放于普通住宅内。
为了您的**和健康,请穿实验服并戴一次性手套操作。