Recombinant Murine PDGF-BB
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产品名称: Recombinant Murine PDGF-BB
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Murine PDGF-BB由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Murine PDGF-BB所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Murine PDGF-BB
的详细介绍
Recombinant Murine PDGF-BB
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产品编号
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产品名称
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产品包装
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产品价格
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XY6130-1mg
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Recombinant
Murine PDGF-BB
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1mg
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20076.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Mouse
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18591
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P31240
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E. coli
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109aa
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24.4kDa
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－
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About this
Antibody
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Name
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Recombinant
Murine PDGF-BB (Recombinant Murine Platelet-derived Growth Factor-BB;
rMuPDGF-BB)；Recombinant Mouse PDGF-BB ; 重组小鼠血小板衍生生长因子-BB
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Synonyms
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Platelet-derived
growth factor subunit B; PDGF subunit B; PDGF-2; Platelet-derived growth
factor B chain; Platelet-derived growth factor beta polypeptide;
Proto-oncogene c-Sis; Pdgfb; Sis
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Purity
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>98% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The ED50 as determined by a
cell proliferation assay using murine Balb/c 3T3 cells is less than 1.0ng/ml,
corresponding to a specific activity of >1.0×106IU/mg.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in PBS, pH 5.0.
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Endotoxin
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Less than
0.1EU/μg of rMuPDGF-BB as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should
be apportioned into working aliquots and stored at ≤ -20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Cytokine
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Background
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Platelet-derived
growth factor is a dimeric glycoprotein composed of two A (-AA) or two B
(-BB) chains or a combination of the two (-AB). All PDGFs function as
secreted, disulphide-linked homodimers, but only PDGF A and B can form
functional heterodimers. PDGFs are mitogenic during early developmental
stages, driving the proliferation of undifferentiated mesenchyme and some
progenitor populations. PDGF is a required element in cellular division for
fibroblasts, a type of connective tissue cell that is especially prevalent in
wound healing. In essence, the PDGFs allow a cell to skip the G1 checkpoints
in order to divide. Recombinant murine PDGF-AA contains 109 amino acids and
has a molecular mass of 12.2kDa.
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Amino Acid
Sequence
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SLGSLAAAEP AVIAECKTRT EVFQISRNLI
DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RASQVQMRPV QVRKIEIVRK KPIFKKATVT LEDHLACKCE
TIVTPRPVT
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包装清单:
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产品编号
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产品名称
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包装
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XY6130-2μg
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Recombinant
Murine PDGF-BB
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2μg
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XY6130-10μg
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Recombinant
Murine PDGF-BB
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10μg
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XY6130-100μg
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Recombinant
Murine PDGF-BB
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100μg
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XY6130-1mg
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Recombinant
Murine PDGF-BB
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。
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