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产品名称: Recombinant Murine bFGF
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Murine bFGF由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Murine bFGF所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Murine bFGF
的详细介绍
Recombinant Murine bFGF
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产品编号
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产品名称
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产品包装
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产品价格
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XY6074-1mg
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Recombinant
Murine bFGF
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1mg
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7206.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Mouse
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14173
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P15655
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E. coli
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146aa
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16.5kDa
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－
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About this
Antibody
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Name
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Recombinant
Murine bFGF (Recombinant Murine Basic Fibroblast Growth Factor; rMubFGF)；Recombinant Mouse bFGF ; 重组小鼠碱性成纤维细胞生长因子
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Synonyms
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FGF2; FGF-2;
FGF2AS; GFG1; HBGH-2; NUDT6; Prostatropin; Basic fibroblast growth factor;
basic fibroblast growth factor bFGF; bFGF; BFGF; FGFBprostatropin; fibroblast
growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2
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Purity
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>98% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The ED50 as determined by a
cell proliferation assay using murine balb/c 3T3 cells is less than 1.0ng/ml,
corresponding to a specific activity of >1.0×106IU/mg.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered solution in PBS, pH7.4.
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Endotoxin
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Less than 1EU/μg of rMubFGF as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Cytokine
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Background
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Murine bFGF,
encoded by the FGF2 gene, is a member of the fibroblast growth factor (FGF)
family. Fibroblast growth factor was found in pituitary extracts in 1973 and
then tested in a bioassay that caused fibroblasts to proliferate. After
further fractionating the extract using acidic and basic pH, two different
forms have isolated that named "acidic fibroblast growth factor"
(FGF-1) and "basic fibroblast growth factor" (FGF-2). Murine bFGF
shares 95% amino acid sequence identity with human bFGF. Affinity between
bFGF and its receptors can be increased by heparin or heparan sulfate
proteoglycan. bFGF plays an important role in the regulation of cell
survival, cell division, angiogenesis, cell differentiation and cell
migration. bFGF are also involved in a variety of biological processes,
including embryonic development , morphogenesis, tissue repair, tumor growth
and invasion. Additionally, bFGF is frequently used for a critical component
of cell culture medium, e.g., human embryonic stem cell culture medium, serum-free
culture systems.
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Amino Acid
Sequence
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MPALPEDGGA AFPPGHFKDP KRLYCKNGGF
FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES
NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS
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包装清单:
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产品编号
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产品名称
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包装
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XY6074-10μg
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Recombinant
Murine bFGF
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10μg
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XY6074-50μg
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Recombinant
Murine bFGF
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50μg
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XY6074-100μg
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Recombinant
Murine bFGF
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100μg
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XY6074-1mg
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Recombinant
Murine bFGF
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。