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产品名称: Recombinant Murine LIF
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Murine LIF由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Murine LIF所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Murine LIF
的详细介绍
Recombinant Murine LIF
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产品编号
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产品名称
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产品包装
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产品价格
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XY6033-1mg
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Recombinant
Murine LIF
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1mg
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14411.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Mouse
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16878
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P09056
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E. coli
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180aa
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19.9kDa
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－
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About this
Antibody
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Name
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Recombinant
Murine LIF (Recombinant Murine Leukemia inhibitory Factor; rMuLIF)；Recombinant Mouse LIF ; 重组小鼠白血病抑制因子
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Synonyms
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CDF; D factor;
DIA; differentiation inhibitory activity; differentiation stimulating factor;
Differentiation-stimulating factor; Emfilermin; HILDAcholinergic
differentiation factor; leukemia inhibitory factor; leukemia inhibitory
factor (cholinergic differentiation factor); Melanoma-derived LPL inhibitor;
MLPLI
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Purity
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>98% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The specific activity is
determined by inducing differentiation of murine M1 myeloid leukemic cells.
The minimum detectable concentration of rMuLIF in this assay is 0.01ng/ml.
The specific activity of >1.0×108IU/mg,
where 50 units is defined as the amount of rMuLIF required to induce
differentiation in 50% of the M1 colonies in 1ml agar cultures.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μM filtered concentrated solution in 20mM PB, pH7.4,
with 0.02% TWEEN 20.
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Endotoxin
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Less than 1EU/μg of rMuLIF as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Cytokine
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Background
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Leukemia
inhibitory factor (LIF) is a member of Interleukin 6 family. This protein is
mainly expressed in the trophectoderm of the developing embryo, with its
receptor LIFR expressed throughout the inner cell mass. LIF has the capacity
to induce terminal differentiation in leukemic cells. Its activities include
the induction of hematopoietic differentiation in normal and myeloid leukemia
cells, the induction of neuronal cell differentiation, and the stimulation of
acute-phase protein synthesis in hepatocytes. LIF is used in mouse embryonic
stem cell culture, because that removal of LIF pushes stem cells toward
differentiation, but they retain their proliferative potential or
pluripotency. It is also used in phase II clinical trial, which can assist
embryo implantation in women who have failed to become pregnant despite
assisted reproductive technologies (ART). Mature mouse LIF shares 78%a.a.
sequence identity with Human LIF.
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Amino Acid
Sequence
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SPLPITPVNA TCAIRHPCHG NLMNQIKNQL
AQLNGSANAL FISYYTAQGE PFPNNVEKLC APNMTDFPSF HGNGTEKTKL VELYRMVAYL SASLTNITRD
QKVLNPTAVS LQVKLNATID VMRGLLSNVL CRLCNKYRVG HVDVPPVPDH SDKEAFQRKK LGCQLLGTYK
QVISVVVQAF
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包装清单:
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产品编号
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产品名称
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包装
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XY6033-5μg
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Recombinant
Murine LIF
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5μg
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XY6033-25μg
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Recombinant
Murine LIF
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25μg
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XY6033-100μg
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Recombinant
Murine LIF
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100μg
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XY6033-1mg
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Recombinant
Murine LIF
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。