Recombinant Murine TNF-α/TNFSF2
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产品名称: Recombinant Murine TNF-α/TNFSF2
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Murine TNF-α/TNFSF2由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Murine TNF-α/TNFSF2所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Murine TNF-α/TNFSF2
的详细介绍
Recombinant Murine TNF-α/TNFSF2
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产品编号
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产品名称
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产品包装
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产品价格
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XY6020-1mg
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Recombinant
Murine TNF-α/TNFSF2
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1mg
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15444.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Mouse
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21926
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P06804
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E. coli
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157aa
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17.4kDa
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－
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About this
Antibody
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Name
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Recombinant
Murine TNF-α/TNFSF2 (Recombinant Murine Tumor Necrosis Factor-α; rMuTNF-α)；Recombinant Mouse TNF-α/TNFSF2 ; 重组小鼠肿瘤坏死因子α
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Synonyms
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Cachetin; DIF;
TNF; tnfa; tnf-a; TNFSF1A; TNFSF2; APC1 protein; Cachectin; DIF; TNF,
monocyte-derived; TNF-a; TNF-alphacachectin; TNFATNF, macrophage-derived;
TNFSF2TNF superfamily, member 2; tumor necrosis factor; tumor necrosis factor
(TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis
factor ligand superfamily member 2; tumor necrosis factor-alpha
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Purity
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>98% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The ED50 as determined by a
cytotoxicity assay using murine L929 cells is less than 0.1ng/ml,
corresponding to a specific activity of >1.0×107IU/mg
in the presence of actinomycin D.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered solution in PBS, pH7.2.
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Endotoxin
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Less than 1EU/μg of rMuTNF-α as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Cytokine
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Background
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Tumor necrosis
factor alpha (TNF-α), also called cachectin, is the best-know member of
the TNF-family, which can cause cell death. This protein is produced by
neutrophils, activated lymphocytes, macrophages, NK cells, LAK cells,
astrocytes endothelial cells, smooth muscle cells and some transformed cells.
TNF-α occurs as a secreted, soluble form and as a
membrane-anchored form, both of which are biologically active. The
naturally-occurring form of TNF-α is glycosylated,
but non-glycosylated recombinant TNF-α has comparable
biological activity. The biologically active native form of TNF-α is reportedly a trimer. Human and murine TNF-α show approximately 79% homology at the amino acid level and
cross-reactivity between the two species. Two types of receptors for TNF-α have been described and virtually all cell types studied show the
presence of one or both of these receptor types.
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Amino Acid
Sequence
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MLRSSSQNSS DKPVAHVVAN HQVEEQLEWL
SQRANALLAN GMDLKDNQLV VPADGLYLVY SQVLFKGQGC PDYVLLTHTV SRFAISYQEK VNLLSAVKSP
CPKDTPEGAE LKPWYEPIYL GGVFQLEKGD QLSAEVNLPK YLDFAESGQV YFGVIAL
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包装清单:
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产品编号
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产品名称
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包装
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XY6020-5μg
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Recombinant
Murine TNF-α/TNFSF2
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5μg
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XY6020-20μg
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Recombinant
Murine TNF-α/TNFSF2
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20μg
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XY6020-100μg
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Recombinant
Murine TNF-α/TNFSF2
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100μg
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XY6020-1mg
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Recombinant
Murine TNF-α/TNFSF2
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。