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产品名称: Recombinant Murine M-CSF
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Murine M-CSF由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Murine M-CSF所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Murine M-CSF
的详细介绍
Recombinant Murine M-CSF
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产品编号
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产品名称
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产品包装
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产品价格
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XY6015-1mg
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Recombinant
Murine M-CSF
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1mg
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22133.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Mouse
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12977
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P07141
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E. coli
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230aa
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52kDa
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－
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About this
Antibody
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Name
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Recombinant
Murine M-CSF (Recombinant Murine Macrophage Colony Stimulating Factor;
rMuM-CSF)；Recombinant Mouse M-CSF ; 重组小鼠巨噬细胞集落刺激因子
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Synonyms
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CSF1; CSF-1;
colony stimulating factor 1 (macrophage); CSF-1; Lanimostim; macrophage
colony stimulating factor; macrophage colony-stimulating factor 1; M-CSF;
MCSFlanimostim; MGC31930
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Purity
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>95% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The ED50 as determined by a
cell proliferation assay using murine M-NFS-60 cells is less than 2ng/ml,
corresponding to a specific activity of >5.0×105IU/mg.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered solution in 20mM Tris, 500mM NaCl, pH7.4.
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Endotoxin
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Less than 1EU/μg of rMuM-CSF as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Cytokine
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Background
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Macrophage
Colony Stimulating Factor (M-CSF), also named CSF-1, is a hematopoietic
growth factor that is involved in the proliferation, differentiation, and
survival of monocytes, macrophages, and bone marrow progenitor cells. It is
produced by osteoblasts (as a result of endocrine stimulation by parathyroid
hormone) exerts paracrine effects on osteoclasts and can interact with CSF1R.
M-CSF is a four α-helical bundle cytokine and its active form is found
extracellularly as a disulfide-linked homodimer. Four transcript variants
encoding three different isoforms have been reported for M-CSF gene. Although
forms may vary, all of them contain the N-terminal 150a.a. portion that is
necessary and sufficient for interaction with the receptor. The first 229a.a.
of mature mouse M-CSF shares 87%, 83%, 82% and 81% sequence identity with
corresponding regions of rat, dog, cow and human M-CSF, respectively. Human
M-CSF is active in the mouse, but mouse M-CSF is reported to be
species-specific.
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Amino Acid
Sequence
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KEVSEHCSHM IGNGHLKVLQ QLIDSQMETS
CQIAFEFVDQ EQLDDPVCYL KKAFFLVQDI IDETMRFKDN TPNANATERL QELSNNLNSC FTKDYEEQNK
ACVRTFHETP LQLLEKIKNF FNETKNLLEK DWNIFTKNCN NSFAKCSSRD VVTKPDCNCL YPKATPSSDP
ASASPHQPPA PSMAPLAGLA WDDSQRTEGS SLLPSELPLR IEDPGSAKQR PPRSTCQTLE
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包装清单:
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产品编号
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产品名称
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包装
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XY6015-2μg
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Recombinant
Murine M-CSF
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2μg
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XY6015-10μg
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Recombinant
Murine M-CSF
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10μg
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XY6015-100μg
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Recombinant
Murine M-CSF
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100μg
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XY6015-1mg
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Recombinant
Murine M-CSF
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。