如果您对该产品感兴趣的话,可以
产品名称: Recombinant Murine TPO
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Murine TPO由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Murine TPO所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Murine TPO
的详细介绍
Recombinant Murine TPO
|
产品编号
|
产品名称
|
产品包装
|
产品价格
|
|
XY6011-1mg
|
Recombinant
Murine TPO
|
1mg
|
22133.00元
|
|
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
|
Mouse
|
21832
|
P40226
|
E. coli
|
174aa
|
18.7kDa
|
－
|
|
About this
Antibody
|
|
Name
|
Recombinant
Murine TPO (Recombinant Murine Thrombopoietin; rMuTPO)；Recombinant Mouse TPO ; 重组小鼠促血小板生成素
|
|
Synonyms
|
MGDF; MK-CSF;
THPO; Tpo; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and
development factor; megakaryocyte stimulating factor; MGDFC-mpl ligand; ML;
MPL ligand; MPLLGMGC163194; Myeloproliferative leukemia virus oncogene
ligand; thrombopoietin; thrombopoietin nirs variant 1; TPOMKCSF
|
|
Purity
|
>95% by
SDS-PAGE and HPLC analyses.
|
|
Biological
Activity
|
Fully
biologically active when compared to standard. The ED50 as determined by a
cell proliferation assay using human MO7e cells is less than 1.0ng/ml,
corresponding to a specific activity of >1.0×106IU/mg.
|
|
Physical
Appearance
|
Sterile
Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized
from a 0.2μm filtered concentrated solution in PBS, pH7.4, with
5% Trehalose.
|
|
Endotoxin
|
Less than
0.1EU/μg of rMuTPO as determined by LAL method.
|
|
Reconstitution
|
We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
|
|
Category
|
Cytokine
|
|
Background
|
Thrombopoietin
(TPO), the ligand for the receptor encoded by the c-Mpl proto-oncogene, is a
key regulator of megakaryocytopoiesis and thrombopoiesis in vitro and in
vivo. The cDNAs for TPO have recently been cloned from canine, murine and
human sources. The proteins from these three species are highly conserved,
exhibiting from 69-75% sequence identity at the amino acid level. Two
distinct domains, separated by a pair of arginine residues that may be a
proteolytic cleavage site, have been identified in TPO: the amino terminal
region exhibiting sequence homology to erythropoietin and the carboxy
terminal region containing multiple potential N-linked glycosylation sites.
Recombinant TPO has now been shown to stimulate the maturation, as well as
the proliferation, of megakaryocytes and may have important therapeutic
applications for the treatment of various clinical conditions associated with
thrombocytopenia.
|
|
Amino Acid
Sequence
|
SPVAPACDPR LLNKLLRDSH LLHSRLSQCP
DVDPLSIPVL LPAVDFSLGE WKTQTEQSKA QDILGAVSLL LEGVMAARGQ LEPSCLSSLL GQLSGQVRLL
LGALQGLLGT QLPLQGRTTA HKDPNALFLS LQQLLRGKVR FLLLVEGPTL CVRRTLPTTA VPSSTSQLLT
LNKF
|
包装清单:
|
产品编号
|
产品名称
|
包装
|
|
XY6011-2μg
|
Recombinant
Murine TPO
|
2μg
|
|
XY6011-10μg
|
Recombinant
Murine TPO
|
10μg
|
|
XY6011-100μg
|
Recombinant
Murine TPO
|
100μg
|
|
XY6011-1mg
|
Recombinant
Murine TPO
|
1mg
|
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。