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产品名称: Recombinant Murine G-CSF
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Murine G-CSF由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Murine G-CSF所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Murine G-CSF
的详细介绍
Recombinant Murine G-CSF
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产品编号
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产品名称
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产品包装
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产品价格
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XY6002-1mg
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Recombinant
Murine G-CSF
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1mg
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27053.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Mouse
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12985
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P09920
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E. coli
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178aa
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18.9kDa
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－
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About this
Antibody
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Name
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Recombinant
Murine G-CSF (Recombinant Murine Granulocyte Colony Stimulating Factor;
rMuG-CSF)；Recombinant Mouse G-CSF ; 重组小鼠粒细胞集落刺激因子
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Synonyms
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CSF3;
C17orf33; chromosome 17 open reading frame 33; colony stimulating factor 3
(granulocyte); CSF3OS; filgrastim; Filgrastim; G-CSF; GCSFlenograstim;
granulocyte colony-stimulating factor; Lenograstim; MGC45931; pluripoietin; Pluripoietin
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Purity
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>98% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The ED50 as determined by a
cell proliferation assay using murine NFS-60 cells is less than 0.05ng/ml,
corresponding to a specific activity of >2.0×107IU/mg.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in 10mM Sodium
Citrate, pH4.0, 150mM NaCl, 0.01% Tween-20.
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Endotoxin
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Less than
0.1EU/μg of rMuG-CSF as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should
be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Cytokine
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Background
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Granulocyte
colony stimulating factor (G-CSF) is a pleiotropic cytokine. It is mainly
produced by monocytes and macrophages upon activation by endotoxin, TNF-α and IFN-γ. Besides, many other cell types
can secreted this protein after LPS, IL-1 or TNF-α activation, which are fibroblasts, endothelial cells, astrocytes and bone
marrow stromal cells. Various carcinoma cell lines and myeloblastic leukemia
cells can express G-CSF constitutively. G-CSF is cytokine that acts in
hematopoiesis by controlling the production, differentiation and function of
2 related white cell populations of the blood, the granulocytes and the
monocytesmacrophages. In addition, it may function in some adhesion or
recognition events at the cell surface. The murine G-CSF cDNA encodes a 208
amino acid (a.a.) residue precursor protein containing a 30a.a. residue
signal peptide that is proteolytically cleaved to generate the 178a.a.
residue mature protein. Murine G-CSF is 73% identical at the amino acid level
to human G-CSF and the two proteins show species cross-reactivity.
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Amino Acid
Sequence
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VPLVTVSALP PSLPLPRSFL LKSLEQVRKI
QASGSVLLEQ LCATYKLCHP EELVLLGHSL GIPKASLSGC SSQALQQTQC LSQLHSGLCL YQGLLQALSG
ISPALAPTLD LLQLDVANFA TTIWQQMENL GVAPTVQPTQ SAMPAFTSAF QRRAGGVLAI SYLQGFLETA
RLALHHLA
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包装清单:
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产品编号
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产品名称
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包装
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XY6002-2μg
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Recombinant
Murine G-CSF
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2μg
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XY6002-10μg
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Recombinant
Murine G-CSF
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10μg
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XY6002-100μg
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Recombinant
Murine G-CSF
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100μg
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XY6002-1mg
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Recombinant
Murine G-CSF
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。