Recombinant Murine IL-1RA
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产品名称: Recombinant Murine IL-1RA
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Murine IL-1RA由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Murine IL-1RA所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Murine IL-1RA
的详细介绍
Recombinant Murine IL-1RA
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产品编号
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产品名称
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产品包装
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产品价格
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XY5903-1mg
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Recombinant
Murine IL-1RA
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1mg
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5718.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Mouse
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16181
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P25085
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E. coli
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152aa
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17.3kDa
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－
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About this
Protein
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Name
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Recombinant
Murine IL-1RA (Recombinant Murine Interleukin-1 Receptor Antagonist Protein;
rMuIL-1RA)；Recombinant Mouse IL-1RA ; 重组小鼠白细胞介素1受体拮抗剂
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Synonyms
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IL-1F3; IL1RN;
DIRA; ICIL-1RA; IL1F3; IL1RA; IL-1ra; IL-1ra3; IL1RN; IL-1RN; interleukin 1
receptor antagonist; IRAP; MVCD4
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Purity
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>97% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The ED50 as determined by
inhibiting IL-1α-dependent proliferation of murine D10S cells is less
than 50ng/ml, corresponding to a specific activity of >2.0×104IU/mg in the presence of 50pg/ml rHuIL-1α.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in PBS, pH7.4.
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Endotoxin
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Less than 1EU/μg of rMuIL-1RA as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should
be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Cytokine
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Background
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IL-1RA was
initially called the IL-1 inhibitor which is encoded by the IL1RN gene and it
is a member of the interleukin 1 cytokine family. IL-1RA is secreted by
various types of cells including immune cells, epithelial cells, and
adipocytes. IL-RA has functions of inhibiting the activity of interleukin-1
by binding to receptor IL1R1 and preventing its association with the
coreceptor IL1RAP for signaling. IL-1RA is also used in the treatment of
rheumatoid arthritis, an autoimmune disease in which IL-1 plays a key role.
The murine IL-1RA is a single non-glycosylated polypeptide chain containing
152 amino acids and it has been shown to block the inflammatory responses
induced by IL-1 both in vitro and in vivo. The protein shows 26% amino acid
homology to IL-1β and 19% homology to IL-1α. It
also shares 90%a.a. sequence identity with rat IL-1RA.
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Amino Acid
Sequence
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RPSGKRPCKM QAFRIWDTNQ KTFYLRNNQL
IAGYLQGPNI KLEEKIDMVP IDLHSVFLGI HGGKLCLSCA KSGDDIKLQL EEVNITDLSK NKEEDKRFTF
IRSEKGPTTS FESAACPGWF LCTTLEADRP VSLTNTPEEP LIVTKFYFQE DQ
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包装清单:
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产品编号
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产品名称
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包装
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XY5903-20μg
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Recombinant
Murine IL-1RA
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20μg
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XY5903-100μg
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Recombinant
Murine IL-1RA
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100μg
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XY5903-1mg
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Recombinant
Murine IL-1RA
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。