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产品名称: Recombinant Human Noggin
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Human Noggin由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Human Noggin所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Human Noggin
的详细介绍
Recombinant Human Noggin
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产品编号
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产品名称
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产品包装
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产品价格
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XY5786-1mg
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Recombinant
Human Noggin
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1mg
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20076.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Human
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9241
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Q13253
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E. coli
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206aa
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46.3kDa
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－
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About this
Antibody
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Name
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Recombinant
Human Noggin (Recombinant Human Noggin; rHuNoggin)；重组人头蛋白
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Synonyms
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NOG; SYM1;
SYNS1; noggin; SYM1; symphalangism 1 (proximal); synostoses (multiple)
syndrome 1; SYNS1
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Purity
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>95% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The ED50 as determined by
inhibiting BMP-4-induced alkaline phosphatase production of murine ATDC5
cells is less than 3.0ng/ml, corresponding to a specific activity of >3.3×105IU/mg in the presence of 5ng/ml rHuBMP-4.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μM filtered concentrated solution in 30% acetonitrile,
0.1% TFA.
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Endotoxin
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Less than 1EU/μg of rHuNoggin as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in 10mM HAc to a concentration of 0.1-1.0mg/ml.
Stock solutions should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Cytokine
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Background
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Noggin encoded
by the NOG gene, was first isolated from Xenopus, having the function of
inducing secondary axis formation in frog embryos. It inhibits TGF-β family ligands and preventing them from binding to their
corresponding receptors. Noggin was originally found as a BMP-4 antagonist,
and then has been shown to modulate the activities of other BMPs (BMP-2, 7,
13 and 14). Additionally, it has pleiotropic effect, both in early
development and later stages. The results of the mouse knockout of noggin suggest
that it is involved in numerous developmental processes, such as neural tube
fusion and joint formation. In recent report, proximal symphalangism (SYM1)
and multiple synostoses syndrome (SYNS1) have relation with the mutant of
evolutionarily conserved amino acid residues of Noggin. Mature human Noggin
shares 99%, 99%, 98%, 97% and 89%a.a. sequence identity with mouse, rat,
bovine, equine and chicken Noggin, respectively.
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Amino Acid
Sequence
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MQHYLHIRPA PSDNLPLVDL IEHPDPIFDP
KEKDLNETLL RSLLGGHYDP GFMATSPPED RPGGGGGAAG GAEDLAELDQ LLRQRPSGAM PSEIKGLEFS
EGLAQGKKQR LSKKLRRKLQ MWLWSQTFCP VLYAWNDLGS RFWPRYVKVG SCFSKRSCSV PEGMVCKPSK
SVHLTVLRWR CQRRGGQRCG WIPIQYPIIS ECKCSC
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包装清单:
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产品编号
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产品名称
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包装
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XY5786-5μg
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Recombinant
Human Noggin
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5μg
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XY5786-20μg
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Recombinant
Human Noggin
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20μg
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XY5786-100μg
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Recombinant
Human Noggin
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100μg
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XY5786-1mg
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Recombinant
Human Noggin
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。