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产品名称: Recombinant Human OPG
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Human OPG由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Human OPG所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Human OPG
的详细介绍
Recombinant Human OPG
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产品编号
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产品名称
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产品包装
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产品价格
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XY5777-1mg
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Recombinant
Human OPG
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1mg
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7722.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Human
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4982
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O00300
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E. coli
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173aa
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19.7kDa
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－
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About this
Antibody
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Name
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Recombinant
Human OPG (Recombinant Human Osteoprotegerin; rHuOPG)；重组人骨保护素
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Synonyms
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OCIF;
TNFRSF11B; OCIFMGC29565; OPGtumor necrosis factor receptor superfamily member
11B; Osteoclastogenesis inhibitory factor; Osteoprotegerin; osteoprotegerin;
TR1; tumor necrosis factor receptor superfamily, member 11b
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Purity
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>95% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The ED50 as determined by
neutralizing the stimulation of U937 cells is less than 10ng/ml,
corresponding to a specific activity of >1.0×105IU/mg
in the presence of 10ng/ml soluble rHuRANKL (sRANKL).
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in 20mM PB,150mM
NaCl, pH6.0.
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Endotoxin
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Less than 1EU/μg of rHuOPG as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Cytokine
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Background
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Osteoprotegerin
(OPG), also named osteoclastogenesis inhibitory factor (OCIF), and tumor
necrosis factor receptor superfamily member 11B (TNFRSF11B), is a
TNFRSF11B-encoded protein in humans. OPG is a 401a.a. basic glycoprotein
which comprises 7 structural domains. It is either a 60kDa monomer or a
120kDa dimer linked by disulfide bridges. OPG acts as a decoy receptor for
the receptor activator of nuclear factor kappa B ligand (RANKL) and inhibits
the activation of osteoclasts and promotes osteoclast apoptosis in vitro and
may also play a role in preventing arterial calcification. OPG has been
applied to decrease bone resorption in women with postmenopausal osteoporosis
and in patients with lytic bone metastases. Mature human OPG shares 86%, 87%,
92%, 92% and 88% amino acid sequence identity with mouse, rat, equine, canine
and bovine OPG, respectively.
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Amino Acid
Sequence
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ETFPPKYLHY DEETSHQLLC DKCPPGTYLK
QHCTAKWKTV CAPCPDHYYT DSWHTSDECL YCSPVCKELQ YVKQECNRTH NRVCECKEGR YLEIEFCLKH
RSCPPGFGVV QAGTPERNTV CKRCPDGFFS NETSSKAPCR KHTNCSVFGL LLTQKGNATH DNICSGNSES
TQK
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包装清单:
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产品编号
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产品名称
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包装
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XY5777-10μg
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Recombinant
Human OPG
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10μg
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XY5777-50μg
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Recombinant
Human OPG
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50μg
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XY5777-100μg
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Recombinant
Human OPG
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100μg
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XY5777-1mg
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Recombinant
Human OPG
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。