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产品名称: Recombinant Human PTN
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Human PTN由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。Recombinant Human PTN所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Human PTN
的详细介绍
Recombinant Human PTN
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产品编号
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产品名称
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产品包装
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产品价格
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XY5736-1mg
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Recombinant
Human PTN
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1mg
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15444.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Human
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5764
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P21246
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E. coli
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136aa
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15.3kDa
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-
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About this
Antibody
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Name
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Recombinant
Human PTN (Recombinant Human Pleiotrophin; rHuPTN);重组人多效生长因子
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Synonyms
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HARP; HBNF;
HNGF-8; OSF-1; PTN; HARP; HBBM; HB-GAM; HBGF8; HBNF; HBNF1; HBNF-1;
Heparin-binding brain mitogen; NEGF1HBGF-8; neurite growth-promoting factor
1; neurite growth-promoting factor1); OSF-1; Osteoblast-specific factor 1;
pleiotrophin
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Purity
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>96% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The biological activity was
measured by its ability to enhance neurite outgrowth of E16-E18 rat embryonic
cortical neurons, when neurons were plated on 96 well culture plates that had
been pre-coated with 100 μl/well of a solution of 5-10μg/ml rHuPTN.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered solution in PBS, pH7.4.
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Endotoxin
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Less than
0.1EU/μg of rHuPTN as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Cytokine
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Background
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Pleiotrophin,
also named heparin-binding brain mitogen (HBBM), is belonging to the
heparin-binding proteins family and is encoded by the PTN gene in humans.
Pleiotrophin is expressed in an activity-dependent manner in the hippocampus.
Pleiotrophin expression is low in other areas of the ***** brain, but it can
be induced by ischemic insults. The cell surface-expressed nucleolin is a low
affinity receptor for PTN binding to cells and it is also implicated in PTN
entry into cells by an active process. Human pleiotrophin shares above 98%
amino acid sequence identity with bovine, rat and murine.
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Amino Acid
Sequence
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GKKEKPEKKV KKSDCGEWQW SVCVPTSGDC
GLGTREGTRT GAECKQTMKT QRCKIPCNWK KQFGAECKYQ FQAWGECDLN TALKTRTGSL KRALHNAECQ
KTVTISKPCG KLTKPKPQAE SKKKKKEGKK QEKMLD
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包装清单:
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产品编号
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产品名称
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包装
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|
XY5736-5μg
|
Recombinant
Human PTN
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5μg
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XY5736-20μg
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Recombinant
Human PTN
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20μg
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XY5736-100μg
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Recombinant
Human PTN
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100μg
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XY5736-1mg
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Recombinant
Human PTN
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存,至少一年有效。由于蛋白的每次冻融均会引起部分失活,所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液),须分装后-20℃或更低温度冻存,以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用,溶液中的蛋白很容易粘附在管壁上,并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein,如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点,使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用,不得用于临床诊断或**,不得用于食品或药品,不得存放于普通住宅内。
为了您的**和健康,请穿实验服并戴一次性手套操作。