Recombinant Human VEGF121
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产品名称: Recombinant Human VEGF121
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Human VEGF121由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Human VEGF121所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Human VEGF121
的详细介绍
Recombinant Human VEGF121
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产品编号
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产品名称
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产品包装
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产品价格
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XY590-1mg
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Recombinant
Human VEGF121
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1mg
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20076.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Human
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7422
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P15692
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Yeast
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121aa
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14.4~20kDa
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－
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About this
Antibody
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Name
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Recombinant
Human VEGF121 (Recombinant Human Vascular Endothelial Growth Factor 121;
rHuVEGF121)；重组人血管内皮生长因子121
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Synonyms
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VPF; VEGF;
MVCD1
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Purity
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>95% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The ED50 as determined by a
cell proliferation assay using human umbilical vein endothelial cells (HUVEC)
is between 0.2-0.4ng/ml.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
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Endotoxin
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Less than
0.1EU/μg of rHuVEGF121 as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Cytokine
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Background
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Vascular
Endothelial Growth Factor is a sub-family of growth factors produced by
cells, which stimulates vasculogenesis and angiogenesis. VEGF's normal
function is to create new blood vessels during embryonic development, new
blood vessels after injury, muscle following exercise, and new vessels
(collateral circulation) to bypass blocked vessels. Humans express
alternately spliced isoforms of 121, 145, 165, 183, 189, and 206 amino acids
(a.a.) in length. VEGF production can be induced in cells that are not receiving
enough oxygen. VEGF165 appears to be the most abundant and potent isoform,
followed by VEGF121 and VEGF189. Recombinant human VEGF121 contains 121 amino
acids residues and it is a disulfide-linked homodimer. In addition, it shares
88% a.a. with corresponding regions of mouse and rat, 96% with porcine, 95%
with canine, and 93% with feline, equine and bovine VEGF, respectively.
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Amino Acid
Sequence
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APMAEGGGQN HHEVVKFMDV YQRSYCHPIE
TLVDIFQEYP DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN
KCECRPKKDR ARQENCDKPR R
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包装清单:
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产品编号
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产品名称
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包装
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XY590-2μg
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Recombinant
Human VEGF121
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2μg
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XY590-10μg
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Recombinant
Human VEGF121
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10μg
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XY590-100μg
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Recombinant
Human VEGF121
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100μg
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XY590-1mg
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Recombinant
Human VEGF121
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。