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产品名称: Recombinant Human HB-EGF
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Human HB-EGF由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Human HB-EGF所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Human HB-EGF
的详细介绍
Recombinant Human HB-EGF
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产品编号
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产品名称
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产品包装
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产品价格
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XY588-1mg
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Recombinant
Human HB-EGF
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1mg
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7722.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Human
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1839
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Q99075
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E. coli
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86aa
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9.7kDa
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－
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About this
Antibody
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Name
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Recombinant
Human HB-EGF (Recombinant Human Heparin-binding EGF-like Growth Factor;
rHuHB-EGF)；重组人肝素结合表皮生长因子样生长因子
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Synonyms
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Dtr; Dts;
Hegfl; diphtheria toxin receptor (heparin-binding epidermal growth
factor-like growthfactor); DTRHEGFLdiphtheria toxin receptor (heparin-binding
EGF-like growth factor); DTS; DTSF; heparin-binding EGF-like growth factor;
heparin-binding epidermal growth factor; proheparin-binding EGF-like growth
factor
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Purity
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>97% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The ED50 as determined by a
cell proliferation assay using murine Balb/c 3T3 cells is less than 1ng/ml,
corresponding to a specific activity of >1.0×106IU/mg.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in 20mM PB, pH7.4,
130mM NaCl.
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Endotoxin
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Less than 1EU/μg of rHuHB-EGF as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1mg/ml. Stock solutions should be
apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Cytokine
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Background
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Heparin-binding
epidermal growth factor (HB-EGF)-like growth factor (EGF) is found in
cerebral neurons. Its expression is increased after hypoxic or ischemic
injury, which also stimulates neurogenesis. HB-EGF has been implicated as a
participant in a variety of normal physiological processes such as blastocyst
implantation, wound healing, and in pathological processes such as tumor
growth, SMC hyperplasia and atherosclerosis. HB-EGF is an 87 amino acid
mitogenic and chemotactic glycoprotein containing an EGF-like domain with six
conserved cysteine residues. Human HB-EGF shares about 73% and 76%a.a.
sequence identity with murine and rat HB-EGF.
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Amino Acid
Sequence
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DLQEADLDLL RVTLSSKPQA LATPNKEEHG
KRKKKGKGLG KKRDPCLRKY KDFCIHGECK YVKELRAPSC ICHPGYHGER CHGLSL
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包装清单:
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产品编号
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产品名称
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包装
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XY588-10μg
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Recombinant
Human HB-EGF
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10μg
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XY588-50μg
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Recombinant
Human HB-EGF
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50μg
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XY588-100μg
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Recombinant
Human HB-EGF
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100μg
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XY588-1mg
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Recombinant
Human HB-EGF
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。