Recombinant Human Amphiregulin
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产品名称: Recombinant Human Amphiregulin
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Human Amphiregulin由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Human Amphiregulin所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Human Amphiregulin
的详细介绍
Recombinant Human Amphiregulin
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产品编号
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产品名称
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产品包装
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产品价格
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XY583-1mg
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Recombinant
Human Amphiregulin
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1mg
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7722.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Human
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374
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P15514
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E. coli
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98aa
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11.3kDa
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－
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About this
Antibody
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Name
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Recombinant
Human Amphiregulin (Recombinant Human Amphiregulin; rHuAmphiregulin)；重组人双调蛋白
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Synonyms
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AREG;
amphiregulin; AR; AREG; AREGB; Colorectum cell-derived growth factor; CRDGF;
MGC13647; schwannoma-derived growth factor; SDGF
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Purity
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>95% by SDS-PAGE
and HPLC analyses.
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Biological
Activity
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Fully
biologically active when compared to standard. The ED50 as determined by a
cell proliferation assay using murine Balb/c 3T3 cells is between 5-10ng/ml.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in PBS, pH7.4.
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Endotoxin
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Less than 1EU/μg of rHuAmphiregulin as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Cytokine
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Background
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Amphiregulin
is an EGF related growth factor and was originally isolated from the
conditioned media of a PMA-treated MCF-7 human breast carcinoma cell line. It
is mainly expressed numerous carcinoma cell lines and the epithelial cells of
various human tissues including colon, stomach, breast, ovary, kidney, etc.
Synthesized as a transmembrane protein, Amphiregulin's extracellular domain
is proteolytically processed to release the mature protein. There are 6
conserved cysteine residues, which form 3 intramolecular disulfide bonds
essential for biological activity. Amphiregulin signals through the EGF/TGF-a
receptor, and stimulates growth of keratinocytes, epithelial cells and some
fibroblasts. It also inhibits the growth of certain carcinoma cell lines. Mutations
in this encoded protein are associated with a psoriasis-like skin phenotype.
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Amino Acid
Sequence
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SVRVEQVVKP PQNKTESENT SDKPKRKKKG
GKNGKNRRNR KKKNPCNAEF QNFCIHGECK YIEHLEAVTC KCQQEYFGER CGEKSMKTHS MIDSSLSK
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包装清单:
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产品编号
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产品名称
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包装
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XY583-10μg
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Recombinant
Human Amphiregulin
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10μg
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XY583-50μg
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Recombinant
Human Amphiregulin
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50μg
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XY583-100μg
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Recombinant
Human Amphiregulin
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100μg
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XY583-1mg
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Recombinant
Human Amphiregulin
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。