如果您对该产品感兴趣的话,可以
产品名称: Recombinant Human GM-CSF
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Human GM-CSF由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Human GM-CSF所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Human GM-CSF
的详细介绍
Recombinant Human GM-CSF
|
产品编号
|
产品名称
|
产品包装
|
产品价格
|
|
XY286-1mg
|
Recombinant
Human GM-CSF
|
1mg
|
16731.00元
|
|
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
|
Human
|
1437
|
P04141
|
E. coli
|
127aa
|
14.5kDa
|
－
|
|
About this
protein
|
|
Name
|
Recombinant
Human GM-CSF (Recombinant Human Granulocyte-Macrophage Colony Stimulating
Factor; rHuGM-CSF)；重组人粒细胞-巨噬细胞集落刺激因子
|
|
Synonyms
|
CSF2; colony
stimulating factor 2 (granulocyte-macrophage); Colony-stimulating factor;
CSF; GM-CSF; GMCSFgranulocyte-macrophage colony-stimulating factor;
granulocyte-macrophage colony stimulating factor; MGC131935; MGC138897;
Molgramostin; molgramostin; sargramostim; Sargramostim
|
|
Purity
|
>98% by
SDS-PAGE and HPLC analyses.
|
|
Biological
Activity
|
Fully
biologically active when compared to standard. The ED50 as determined by a
cell proliferation assay using human TF-1 cells is less than 0.1ng/ml,
corresponding to a specific activity of >1.0×107IU/mg.
|
|
Physical
Appearance
|
Sterile
Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized
from a 0.2μm filtered concentrated solution in PBS, pH7.4.
|
|
Endotoxin
|
Less than 1EU/μg of rHuGM-CSF as determined by LAL method.
|
|
Reconstitution
|
We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
|
|
Category
|
Cytokine
|
|
Background
|
Granulocyte-Macrophage
Colony Stimulating Factor (GM-CSF) is secreted by a number of different cell
types (including activated T cells, B cells, macrophages, mast cells,
endothelial cells and fibroblasts) in response to cytokine or immune and
inflammatory stimulation. It was initially characterized as a growth factor
that can support the in vitro colony formation of granulocyte-macrophage
progenitors and has functions of stimulates the growth and differentiation of
hematopoietic precursor cells from various lineages. GM-CSF has also been
reported to have a functional role on non-hematopoietic cells and can induce
human endothelial cells to migrate and proliferate. Additionally, it can
stimulate the proliferation of a number of tumor cell lines, including osteogenic
sarcoma, carcinoma and adenocarcinoma cell lines. Human GM-CSF shares 54%
sequences identity with mouse GM-CSF, but has no biological effects across
species. GM-CSF is used as a medication to stimulate the production of white
blood cells following chemotherapy and has also recently been evaluated in
clinical trials for its potential as a vaccine adjuvant in HIV-infected
patients.
|
|
Amino Acid
Sequence
|
APARSPSPST QPWEHVNAIQ EARRLLNLSR
DTAAEMNETV EVISEMFDLQ EPTCLQTRLE LYKQGLRGSL TKLKGPLTMM ASHYKQHCPP TPETSCATQI
ITFESFKENL KDFLLVIPFD CWEPVQE
|
包装清单:
|
产品编号
|
产品名称
|
包装
|
|
XY286-5μg
|
Recombinant
Human GM-CSF
|
5μg
|
|
XY286-20μg
|
Recombinant
Human GM-CSF
|
20μg
|
|
XY286-100μg
|
Recombinant
Human GM-CSF
|
100μg
|
|
XY286-1mg
|
Recombinant
Human GM-CSF
|
1mg
|
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。