如果您对该产品感兴趣的话,可以
产品名称: Recombinant Human IL-10
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Human IL-10由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Human IL-10所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Human IL-10
的详细介绍
Recombinant Human IL-10
|
产品编号
|
产品名称
|
产品包装
|
产品价格
|
|
XY165-1mg
|
Recombinant
Human IL-10
|
1mg
|
26773.00元
|
|
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
|
Human
|
3586
|
P22301
|
E. coli
|
160aa
|
18.6kDa
|
－
|
|
About this
protein
|
|
Name
|
Recombinant
Human IL-10 (Recombinant Human Interleukin-10; rHuIL-10)；重组人白细胞介素10
|
|
Synonyms
|
CSIF; GVHDS;
IL-10; IL10A; TGIF
|
|
Purity
|
>95% by
SDS-PAGE and HPLC analyses.
|
|
Biological
Activity
|
Fully
biologically active when compared to standard. The ED50 as determined by a
cell proliferation assay using murine MC/9-2 cells is less than 1ng/ml,
corresponding to a specific activity of >1.0×106IU/mg.
|
|
Physical
Appearance
|
Sterile
Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized
from a 0.2μm filtered concentrated solution in PBS, pH7.4.
|
|
Endotoxin
|
Less than 1EU/μg of rHuIL-10 as determined by LAL method.
|
|
Reconstitution
|
We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
|
|
Category
|
Cytokine
|
|
Background
|
Interleukin-10
(IL-10), also known as cytokine synthesis inhibitory factor (CSIF), is the
charter member of the IL-10 family of α-helical cytokines
that also includes IL-19, IL-20, IL-22, IL-24, and IL-26/AK155. IL-10 is
secreted by many activated hematopoietic cell types as well as hepatic
stellate cells, keratinocytes, and placental cytotrophoblasts. Whereas human
IL-10 is active on mouse cells, mouse IL-10 does not act on human cells.
IL-10 is a 178 amino acid molecule that contains two intrachain disulfide
bridges and is expressed as a 36kDa noncovalently associated homodimer. The
IL-10 dimer binds to two IL-10Rα/IL-10R1 chains,
resulting in recruitment of two IL-10Rβ/IL-10R2
chains and activation of a signaling cascade involving JAK1, TYK2, and STAT3.
IL-10Rβ does not bind IL-10 by itself but is required
for signal transduction. IL-10 is a critical molecule in the control of viral
infections and allergic and autoimmune inflammation. It promotes phagocytic
uptake and Th2 responses but suppresses antigen presentation and Th1
proinflammatory responses.
|
|
Amino Acid
Sequence
|
SPGQGTQSEN SCTHFPGNLP NMLRDLRDAF
SRVKTFFQMK DQLDNLLLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENQDPDIKAH VNSLGENLKT
LRLRLRRCHR FLPCENKSKA VEQVKNAFNK LQEKGIYKAM SEFDIFINYI EAYMTMKIRN
|
包装清单:
|
产品编号
|
产品名称
|
包装
|
|
XY165-2μg
|
Recombinant
Human IL-10
|
2μg
|
|
XY165-10μg
|
Recombinant
Human IL-10
|
10μg
|
|
XY165-100μg
|
Recombinant
Human IL-10
|
100μg
|
|
XY165-1mg
|
Recombinant
Human IL-10
|
1mg
|
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。