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产品名称: Recombinant Human IL-6
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Human IL-6由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Human IL-6所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Human IL-6
的详细介绍
Recombinant Human IL-6
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产品编号
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产品名称
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产品包装
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产品价格
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XY138-1mg
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Recombinant
Human IL-6
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1mg
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15444.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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Human
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3569
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P05231
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E. coli
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183aa
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20.7kDa
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－
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About this
protein
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Name
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Recombinant
Human IL-6 (Recombinant Human Interleukin-6; rHuIL-6)；重组人白细胞介素6
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Synonyms
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BSF-2;
IFN-beta 2; MGI-2A; B cell stimulatory factor-2; B-cell differentiation
factor; BSF-2; BSF2CTL differentiation factor; CDF; HGFHSFIFNB2Hybridoma
growth factor; IFN-beta-2; IL-6B-cell stimulatory factor 2; Interferon
beta-2; interleukin 6 (interferon, beta 2); interleukin BSF-2; interleukin-6
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Purity
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>96% by
SDS-PAGE and HPLC analyses.
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Biological
Activity
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Assay #1:
Fully biologically active when compared to standard. The ED50 as determined
by a cell proliferation assay using IL-6-dependent murine 7TD1 cells is less
than 0.1ng/ml, corresponding to a specific activity of >1.0×107IU/mg. Assay #2: Fully biologically active when
compared to standard. The ED50 as determined by a cell proliferation assay
using IL-6-dependent murine T1165 cells is less than 0.8ng/ml, corresponding
to a specific activity of >1.25×106IU/mg.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from a 0.2μm filtered concentrated solution in PBS, pH7.4.
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Endotoxin
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Less than
1.0EU/μg of rHuIL-6 as determined by LAL method.
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Reconstitution
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We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions
should be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
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Category
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Cytokine
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Background
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Interleukin-6
(IL-6) is an interleukin that in humans is encoded by the IL-6 gene and acts
as both a pro-inflammatory and antiinflammatory cytokine. It is secreted by T
cells and macrophages to stimulate immune response. Furthermore, It plays an
essential role in the final differentiation of B-cells into Ig-secreting
cells involved in lymphocyte and monocyte differentiation. It also induces
myeloma and plasmacytoma growth and induces nerve cells differentiation acts
on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of
the CNS. The human IL-6 is a single non-glycosylated polypeptide chain
containing 183 amino acids and it signals through a cell-surface type I
cytokine receptor complex consisting of the ligand-binding IL-6Rα chain (CD126), and the signal- transducing component gp130 (also
called CD130). The human IL-6 shares about 40%a.a. sequence identity with
mouse and rat IL-6 and it is equally active on mouse and rat cells.
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Amino Acid
Sequence
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VPPGEDSKDV AAPHRQPLTS SERIDKQIRY ILDGISALRK
ETCNKSNMCE SSKEALAENN LNLPKMAEKD GCFQSGFNEE TCLVKIITGL LEFEVYLEYL QNRFESSEEQ
ARAVQMSTKV LIQFLQKKAK NLDAITTPDP TTNASLLTKL QAQNQWLQDM TTHLILRSFK EFLQSSLRAL
RQM
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包装清单:
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产品编号
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产品名称
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包装
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XY138-5μg
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Recombinant
Human IL-6
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5μg
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XY138-20μg
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Recombinant
Human IL-6
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20μg
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XY138-100μg
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Recombinant
Human IL-6
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100μg
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XY138-1mg
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Recombinant
Human IL-6
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1mg
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。