Recombinant Human IL-1 RA
如果您对该产品感兴趣的话,可以
产品名称: Recombinant Human IL-1 RA
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Human IL-1 RA由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。Recombinant Human IL-1 RA所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Human IL-1 RA
的详细介绍
Recombinant Human IL-1 RA
|
产品编号
|
产品名称
|
产品包装
|
产品价格
|
|
XY111-1mg
|
Recombinant
Human IL-1 RA
|
1mg
|
5665.00元
|
|
Species
|
Gene ID
|
Accession
|
Source
|
Length
|
MW
|
Tag
|
|
Human
|
3557
|
P18510
|
E. coli
|
152aa
|
17.1kDa
|
－
|
|
About this
Protein
|
|
Name
|
Recombinant
Human IL-1 RA (Recombinant Human Interleukin-1 Receptor Antagonist;
rHuIL-1RA)；重组人白细胞介素1受体拮抗剂
|
|
Synonyms
|
IL-1F3; IL1RN;
DIRA; ICIL-1RA; IL1F3; IL1RA; IL-1ra; IL-1ra3; IL1RN; IL-1RN; Interleukin 1
receptor antagonist; IRAP; MVCD4
|
|
Purity
|
>96% by
SDS-PAGE and HPLC analyses
|
|
Biological
Activity
|
Fully
biologically active when compared to standard. The ED50 as determined by
inhibiting IL-1α-dependent proliferation of murine D10S cells is less
than 40ng/ml, corresponding to a specific activity of >2.5×104IU/mg in the presence of 50pg/ml rHuIL-1α.
|
|
Physical
Appearance
|
Sterile
Filtered White lyophilized (freeze-dried) powder.
|
|
Formulation
|
Lyophilized
from a 0.2μm filtered concentrated solution in PBS, pH 7.4.
|
|
Endotoxin
|
Less than 1EU/μg of rHuIL-1RA as determined by LAL method.
|
|
Reconstitution
|
We recommend
that this vial be briefly centrifuged prior to opening to bring the contents
to the bottom. Reconstitute in sterile distilled water or aqueous buffer
containing 0.1% BSA to a concentration of 0.1-1.0mg/ml. Stock solutions should
be apportioned into working aliquots and stored at ≤-20℃. Further dilutions should be made in
appropriate buffered solutions.
|
|
Category
|
Cytokine
|
|
Background
|
Interleukin-1
receptor antagonist (IL-1RA) is a member of the IL-1 family. IL-1RA is
secreted by various types of cells including immune cells, epithelial cells,
and adipocytes, and is a natural inhibitor of the pro-inflammatory effect of
IL-1β. This protein inhibits the activities of interleukin
1, alpha (IL-1α) and interleukin 1, beta (IL-1β), and modulates a variety of interleukin 1 related immune and
inflammatory responses. IL-4 and IL-13 have been shown to amplify the
stimulatory effect of IL1-beta on the production of soluble and intracellular
forms of IL1-RA. The regulated expression of IL-1RA in various cell types has
been shown to be influenced by cytokines. In synovial fibroblasts the
synthesis of IL-1RA is markedly enhanced by IL-1, TNF-α, or PDGF. The protein shows 26% amino acid homology to IL-1β and 19% homology to IL-1α.
|
|
Amino Acid
Sequence
|
RPSGRKSSKM QAFRIWDVNQ KTFYLRNNQL
VAGYLQGPNV NLEEKIDVVP IEPHALFLGI HGGKMCLSCV KSGDETRLQL EAVNITDLSE NRKQDKRFAF
IRSDSGPTTS FESAACPGWF LCTAMEADQP VSLTNMPDEG VMVTKFYFQE DE
|
包装清单:
|
产品编号
|
产品名称
|
包装
|
|
XY111-20μg
|
Recombinant
Human IL-1 RA
|
20μg
|
|
XY111-100μg
|
Recombinant
Human IL-1 RA
|
100μg
|
|
XY111-1mg
|
Recombinant
Human IL-1 RA
|
1mg
|
|
-
|
说明书
|
1份
|
保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。