Species

Gene ID

Accession

Source

Length

MW

Tag

－

－

－

E. coli

430aa

47.8kDa

－

About this Protein

Name

Recombinant Cys-Protein A/G (Recombinant Cys-Protein A/G; Cys-Protein A/G)；重组蛋白A/G，N端带Cys

Synonyms

N/A

Purity

>96% by SDS-PAGE and RP-HPLC analyses.

Biological Activity

The recombinant Protein A/G is a genetically engineered protein containing 5 IgG-binding regions of protein A and 2 of protein G. Cell wall binding region, cell membrane binding region and albumin binding region have been removed from the recombinant Protein A/G to ensure the maximum specific IgG binding. The recombinant Protein A/G is ideal for purification of polyclonal or monoclonal IgG antibodies. Protein A/G binds to various human, mouse and rat IgG subclasses (e.g., human IgG1, IgG2, IgG3, IgG4; mouse IgG2a, IgG2b, IgG3; rat IgG2a, IgG2c). It also binds to total IgG from cow, goat, sheep, house, rabbit, guinea pig, pig, dog and cat.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from additive free solution.

Endotoxin

Less than 0.1EU/μg of Cys-Protein A/G as determined by LAL method.

Reconstitution

Dissolve in distilled water or saline.

Category

Protein A/G

Background

The recombinant Protein A/G consists of 7 IgG-binding domains EDABC-C1C3, which corresponds to the Protein A and G domains that are included in the recombinant sequence. The Protein A portion is from Staphylococcus aureus segments E, D, A, B and C. The Protein G portion is from Streptococcus segments C1 and C3. The binding capacity of recombinant Protein A/G is broader than either Protein A or Protein G alone.

Amino Acid Sequence

CNAAQHDEAQ QNAFYQVLNM PNLNADQRNG FIQSLKDDPS QSANVLGEAQ KLNDSQAPKA DAQQNNFNKD QQSAFYEILN MPNLNEAQRN GFIQSLKDDP SQSTNVLGEA KKLNESQAPK ADNNFNKEQQ NAFYEILNMP NLNEEQRNGF IQSLKDDPSQ SANLLSEAKK LNESQAPKAD NKFNKEQQNA FYEILHLPNL NEEQRNGFIQ SLKDDPSQSA NLLAEAKKLN DAQAPKADNK FNKEQQNAFY EILHLPNLTE EQRNGFIQSL KDDPSVSKEI LAEAKKLNDA QAPKEEDSLE GSGSGTYKLI LNGKTLKGET TTEAVDAATA EKVFKQYAND NGVDGEWTYD DATKTFTVTE KPEVIDASEL TPAVTTYKLV INGKTLKGET TTKAVDAETA EKAFKQYAND NGVDGVWTYD DATKTFTVTE

产品编号

产品名称

包装

XY052-1mg

Recombinant Cys-Protein A/G

1mg

XY052-5mg

Recombinant Cys-Protein A/G

5mg

XY052-1g

Recombinant Cys-Protein A/G

1g

-

说明书

1份