Recombinant Protein A, His-tag
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产品名称: Recombinant Protein A, His-tag
产品型号:
产品展商: XYbscience
产品文档: 无相关文档
简单介绍
Recombinant Protein A, His-tag由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。Recombinant Protein A, His-tag而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
Recombinant Protein A, His-tag
的详细介绍
Recombinant Protein A, His-tag
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产品编号
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产品名称
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产品包装
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产品价格
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XY5003-1g
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Recombinant
Protein A, His-tag
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1g
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5218.00元
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Species
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Gene ID
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Accession
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Source
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Length
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MW
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Tag
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－
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－
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－
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E. coli
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306aa
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34.7kDa
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His
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About this
Protein
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Name
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Recombinant
Protein A, His-tag (Recombinant Protein A, His-tag; Protein A, His-tag)；重组蛋白A，His标签
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Synonyms
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N/A
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Purity
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>97% by
SDS-PAGE and HPLC analyses
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Biological
Activity
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The
recombinant Protein A is a genetically engineered protein containing 5
IgG-binding regions of protein A. Cell wall binding region, cell membrane
binding region and albumin binding region have been removed from the
recombinant Protein A to ensure the maximum specific IgG binding. The
recombinant Protein A is ideal for purification of polyclonal or monoclonal
IgG antibodies. Protein A binds to various human, mouse and rat IgG
subclasses (e.g., human IgG1, IgG2, IgG4; mouse IgG2a, IgG2b, IgG3; rat
IgG2c). It also binds to total IgG from rabbit, guinea pig, pig, dog and cat.
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Physical
Appearance
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Sterile
Filtered White lyophilized (freeze-dried) powder.
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Formulation
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Lyophilized
from additive free solution.
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Endotoxin
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Less than
0.1EU/μg of Protein A, His-tag as determined by LAL method.
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Reconstitution
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Dissolve in
distilled water or saline.
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Category
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Protein A
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Background
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Protein A is a
cell wall component produced by several strains of Staphylococcus aureus that
consists of a single polypeptide chain and contains little or no
carbohydrate. Recombinant Protein A is produced in E. Coli and functions
essentially the same as native Protein A. It consists of 5 IgG-binding
domains E-D-A-B-C aligned in series.
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Amino Acid
Sequence
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MNAAQHDEAQ QNAFYQVLNM PNLNADQRNG
FIQSLKDDPS QSANVLGEAQ KLNDSQAPKA DAQQNNFNKD QQSAFYEILN MPNLNEAQRN GFIQSLKDDP
SQSTNVLGEA KKLNESQAPK ADNNFNKEQQ NAFYEILNMP NLNEEQRNGF IQSLKDDPSQ SANLLSEAKK
LNESQAPKAD NKFNKEQQNA FYEILHLPNL NEEQRNGFIQ SLKDDPSQSA NLLAEAKKLN DAQAPKADNK
FNKEQQNAFY EILHLPNLTE EQRNGFIQSL KDDPSVSKEI LAEAKKLNDA QAPKEEDSLE HHHHHH
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包装清单:
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产品编号
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产品名称
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包装
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XY5003-10mg
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Recombinant
Protein A, His-tag
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10mg
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XY5003-100mg
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Recombinant
Protein A, His-tag
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100mg
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XY5003-1g
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Recombinant
Protein A, His-tag
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1g
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-
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说明书
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1份
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保存条件:
-20℃或更低温度保存，至少一年有效。由于蛋白的每次冻融均会引起部分失活，所以**配制成相应浓度的储存液后(请根据产品简介中Reconstitution一栏的信息配制储存液)，须分装后-20℃或更低温度冻存，以避免反复冻融。
注意事项:
由于有些塑料管壁对某些蛋白有较强的吸附作用，溶液中的蛋白很容易粘附在管壁上，并且粘附后的蛋白很难与管壁分离。而载体蛋白(Carrier protein，如0.1% BSA等)的主要作用是预先封闭塑料管壁上的蛋白结合位点，使细胞因子或重组蛋白不会粘附于管壁。所以一定要使用产品简介中Reconstitution一栏的信息配制储存液。
本产品**于专业人员的科学研究用，不得用于临床诊断或**，不得用于食品或药品，不得存放于普通住宅内。
为了您的**和健康，请穿实验服并戴一次性手套操作。