质粒序列
LOCUS Exported 3023 bp ds-DNA circular SYN 12-SEP-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3023)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Monday, September 12, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..3023
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 36..415
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 416..619
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 664..682
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 792..815
/codon_start=1
/product="FLAG(R) epitope tag, followed by an enterokinase
cleavage site"
/note="FLAG"
/translation="DYKDDDDK"
polyA_signal 853..1077
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
CDS 1250..2044
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
rep_origin 2370..2958
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gactcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga ctagttatta
61 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
121 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
181 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
241 ctatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
301 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
361 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
421 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
481 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
541 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
601 ggtctatata agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga
661 aattaatacg actcactata gggagaccca agctggctag cgtttaaact taagcttggt
721 accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcca gcacagtggc
781 ggccgctcga ggactacaag gacgacgatg acaagtctag agggcccgtt taaacccgct
841 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
901 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
961 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
1021 agggggagga ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt
1081 ctactgggcg gttttatgga cagcaagcga accggaattg ccagctgggg cgccctctgg
1141 taaggttggg aagccctgca aagtaaactg gatggctttc tcgccgccaa ggatctgatg
1201 gcgcagggga tcaagctctg atcaagagac aggatgagga tcgtttcgca tgattgaaca
1261 agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg
1321 ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg
1381 cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc
1441 agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt
1501 cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc
1561 atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca
1621 tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc
1681 acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg
1741 gctcgcgcca gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct
1801 cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc
1861 tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc
1921 tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta
1981 cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt
2041 ctgaattatt aacgcttaca atttcctgat gcggtatttt ctccttacgc atctgtgcgg
2101 tatttcacac cgcatacagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
2161 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
2221 cttcaataat agcacgtgct aaaacttcat ttttaattta aaaggatcta ggtgaagatc
2281 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
2341 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
2401 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
2461 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
2521 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
2581 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
2641 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
2701 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
2761 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
2821 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
2881 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
2941 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct gggcttttgc
3001 tggccttttg ctcacatgtt ctt
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