质粒序列
LOCUS Exported 3415 bp ds-DNA circular SYN 03-DEC-2013
DEFINITION Bacterial expression vector with a T5 promoter. For other reading
frames, use pQE-3 or pQE-5.
ACCESSION .
VERSION .
KEYWORDS pQE-4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3415)
AUTHORS Qiagen
TITLE Direct Submission
JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
COMMENT Discontinued pQE vector.
FEATURES Location/Qualifiers
source 1..3415
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
promoter 10..54
/note="T5 promoter"
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 30..46
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
protein_bind 62..78
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 101..106
/note="ribosome binding site"
CDS 115..117
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
misc_feature 120..145
/note="MCS"
/note="multiple cloning site"
misc_feature 145..155
/note="stop codons"
/note="stop codons in all three reading frames"
terminator 161..255
/note="lambda t0 terminator"
/note="transcription terminator from phage lambda"
CDS 299..958
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
terminator 1023..1109
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin complement(1591..2179)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2350..3210)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQAAMDERNRQIAEIGAS
LIKHW"
promoter complement(3211..3315)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgagg
121 gatccgtcga cctgcagcca agcttaatta gctgagcttg gactcctgtt gatagatcca
181 gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt
241 tttattggtg agaatccaag ctagcttggc gagattttca ggagctaagg aagctaaaat
301 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca
361 ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat
421 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca
481 cattcttgcc cgcctgatga atgctcatcc ggaatttcgt atggcaatga aagacggtga
541 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
601 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
661 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
721 tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
781 caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga
841 caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtctgtgatg gcttccatgt
901 cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt
961 tttttaaggc agttattggt gcccttaaac gcctggggta atgactctct agcttgaggc
1021 atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt
1081 cggtgaacgc tctcctgagt aggacaaatc cgccgctcta gagctgcctc gcgcgtttcg
1141 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt
1201 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc
1261 ggggcgcagc catgacccag tcacgtagcg atagcggagt gtatactggc ttaactatgc
1321 ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg
1381 cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg
1441 ctcggtctgt cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc
1501 cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag
1561 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
1621 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
1681 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
1741 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag
1801 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
1861 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
1921 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
1981 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt
2041 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
2101 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
2161 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
2221 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
2281 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
2341 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
2401 ttcatccata gctgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
2461 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
2521 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
2581 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
2641 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
2701 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
2761 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
2821 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
2881 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
2941 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
3001 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
3061 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
3121 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
3181 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
3241 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
3301 aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtctaag aaaccattat
3361 tatcatgaca ttaacctata aaaataggcg tatcacgagg ccctttcgtc ttcac
//