质粒序列
LOCUS Exported 4004 bp ds-DNA circular SYN 05-DEC-2013
DEFINITION Bacterial vector for expressing DHFR fusion proteins. Similar to
pQE-18, but with a different reading frame after the DHFR.
ACCESSION .
VERSION .
KEYWORDS pQE-17
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4004)
AUTHORS Qiagen
TITLE Direct Submission
JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
COMMENT Discontinued pQE vector.
FEATURES Location/Qualifiers
source 1..4004
/organism="synthetic DNA construct"
/lab_host="Escherichia coli"
/mol_type="other DNA"
promoter 10..54
/note="T5 promoter"
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 30..46
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
protein_bind 62..78
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 101..106
/note="ribosome binding site"
CDS 115..117
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
CDS 133..690
/codon_start=1
/gene="Mus musculus Dhfr"
/product="mouse dihydrofolate reductase"
/note="DHFR"
/translation="MVRPLNSIVAVSQNMGIGKNGDLPWPPLRNEFKYFQRMTTTSSVE
GKQNLVIMGRKTWFSIPEKNRPLKDRINIVLSRELKEPPRGAHFLAKSLDDALRLIEQP
ELASKVDMVWIVGGSSVYQEAMNQPGHLRLFVTRIMQEFESDTFFPEIDLGKYKLLPEY
PGVLSEVQEEKGIKYKFEVYEKK"
CDS 710..727
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
misc_feature 734..744
/note="stop codons"
/note="stop codons in all three reading frames"
terminator 750..844
/note="lambda t0 terminator"
/note="transcription terminator from phage lambda"
CDS 888..1547
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
terminator 1612..1698
/gene="Escherichia coli rrnB"
/note="rrnB T1 terminator"
/note="transcription terminator T1 from the E. coli rrnB
gene"
rep_origin complement(2180..2768)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(2939..3799)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQAAMDERNRQIAEIGAS
LIKHW"
promoter complement(3800..3904)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 ctcgagaaat cataaaaaat ttatttgctt tgtgagcgga taacaattat aatagattca
61 attgtgagcg gataacaatt tcacacagaa ttcattaaag aggagaaatt aactatgaga
121 ggatccggca tcatggttcg accattgaac tcgatcgtcg ccgtgtccca aaatatgggg
181 attggcaaga acggagacct accctggcct ccgctcagga acgagttcaa gtacttccaa
241 agaatgacca caacctcttc agtggaaggt aaacagaatc tggtgattat gggtaggaaa
301 acctggttct ccattcctga gaagaatcga cctttaaagg acagaattaa tatagttctc
361 agtagagaac tcaaagaacc accacgagga gctcattttc ttgccaaaag tttggatgat
421 gccttaagac ttattgaaca accggaattg gcaagtaaag tagacatggt ttggatagtc
481 ggaggcagtt ctgtttacca ggaagccatg aatcaaccag gccaccttag actctttgtg
541 acaaggatca tgcaggaatt tgaaagtgac acgtttttcc cagaaattga tttggggaaa
601 tataaacttc tcccagaata cccaggcgtc ctctctgagg tccaggagga aaaaggcatc
661 aagtataagt ttgaagtcta cgagaagaaa ggttccagat cttcgatctc atcaccatca
721 ccatcactaa gcttaattag ctgagcttgg actcctgttg atagatccag taatgacctc
781 agaactccat ctggatttgt tcagaacgct cggttgccgc cgggcgtttt ttattggtga
841 gaatccaagc tagcttggcg agattttcag gagctaagga agctaaaatg gagaaaaaaa
901 tcactggata taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat
961 ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt
1021 taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc
1081 gcctgatgaa tgctcatccg gaatttcgta tggcaatgaa agacggtgag ctggtgatat
1141 gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc
1201 tctggagtga ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg
1261 cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg
1321 tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca
1381 acttcttcgc ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga
1441 tgccgctggc gattcaggtt catcatgccg tctgtgatgg cttccatgtc ggcagaatgc
1501 ttaatgaatt acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca
1561 gttattggtg cccttaaacg cctggggtaa tgactctcta gcttgaggca tcaaataaaa
1621 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
1681 ctcctgagta ggacaaatcc gccgctctag agctgcctcg cgcgtttcgg tgatgacggt
1741 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
1801 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc
1861 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc
1921 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
1981 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtctgtc
2041 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
2101 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
2161 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
2221 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
2281 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
2341 cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt
2401 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
2461 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
2521 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
2581 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
2641 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
2701 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
2761 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
2821 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
2881 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
2941 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
3001 ctgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
3061 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
3121 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
3181 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
3241 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
3301 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
3361 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
3421 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
3481 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
3541 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
3601 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
3661 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
3721 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
3781 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
3841 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
3901 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
3961 taacctataa aaataggcgt atcacgaggc cctttcgtct tcac
//