质粒序列
LOCUS Exported 5250 bp ds-DNA circular SYN 05-MAY-2014
DEFINITION Vector for expressing untagged proteins in insect and mammalian
cells using the QIAgenes system.
ACCESSION .
VERSION .
KEYWORDS pQE-TriSystem-7
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5250)
AUTHORS Qiagen
TITLE Direct Submission
JOURNAL Exported Wednesday, September 14, 2016 from SnapGene Viewer 3.1.4
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..5250
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_recomb 181..1008
/note="baculovirus recombination region (lef2/ORF603)"
/note="contains ORF603 and part of lef2"
CDS 181..305
/codon_start=3
/product="baculovirus late expression factor 2"
/note="lef2"
/translation="HLLIKSQDVYKPPNCQKMKTVDKLCPFAGNCKGLNPICNY"
CDS complement(343..948)
/codon_start=1
/product="baculovirus ORF603 protein"
/note="ORF603"
/translation="MAVIFNNKQLLADNSIEKGGELFLFNGSYNILESYVNPVLLKNGV
IELEEAAYYAGNILYKTDDPKFIDYINLIIKATHSEELPENSTVVNYRKTMRSGTIHPI
KKDIYIYDNKKFTLYDRYIYGYDNNYVNFYEEKNEKEKEYEEEDDKASSLCENKIILSQ
INCESFENDFKYYLSDYNYAFSIIDNTTNVLVAFGLYR"
enhancer 1138..1443
/note="CMV enhancer"
/note="human cytomegalovirus (CMV) immediate early
enhancer"
promoter 1449..1726
/note="chicken beta-actin promoter"
exon 1727..1819
/note="Exon 1"
intron 1820..2081
exon 2082..2634
/note="Exon 2"
promoter 2150..2168
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 2169..2193
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter 2209..2318
/note="p10 promoter"
/note="baculovirus promoter for expression in insect cells"
RBS 2321..2326
/note="ribosome binding site"
misc_feature 2331..2404
/note="MCS"
/note="multiple cloning site"
CDS 2333..2335
/codon_start=1
/product="start codon"
/note="ATG"
/translation="M"
misc_feature 2407..2425
/note="siRNA target site"
CDS 2432..2455
/codon_start=1
/product="8xHis affinity tag"
/note="8xHis"
/translation="HHHHHHHH"
misc_feature 2456..2466
/note="stop codons"
/note="stop codons in all three reading frames"
polyA_signal 2610..2615
terminator 2632..2743
/note="rabbit globin terminator"
terminator 2753..2800
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
misc_feature 2813..3518
/note="baculovirus recombination region (ORF1629)"
/note="contains part of ORF1629"
CDS complement(2930..3518)
/codon_start=2
/product="baculovirus capsid-associated protein"
/note="ORF1629"
/note="required for viral replication"
/translation="IPKSSTTNLIADVLADTINRRRVAMAKSSSEATSNDEGWDDDDNR
PNKANTPDVKYVQALFNVFTSSQLYTNDSDERNTKAHNILNDVEPLLQNKTQTNIDKAR
LLLQDLASFVALSENPLDSPAIGSEKQPLFETNRNLFYKSIEDLIFKFRYKDAENHLIF
ALTYHPKDYKFNELLKYVQQLSVNQQRTESSA"
rep_origin complement(3728..4315)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4328..5188)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
ORIGIN
1 tcctgcatct tttaatcaaa tcccaagatg tgtataaacg cgccggtatg tacaggaaga
61 ggtttatact aaactgttac attgcaaacg tggtttcgtg tgccaagtgt gaaaaccgat
121 gtttaatcaa ggctctgacg catttctaca accacgactc caagtgtgtg ggtgaagtca
181 tgcatctttt aatcaaatcc caagatgtgt ataaaccacc aaactgccaa aaaatgaaaa
241 ctgtcgacaa gctctgtccg tttgctggca actgcaaggg tctcaatcct atttgtaatt
301 attgaataat aaaacaatta taaatgtcaa atttgttttt tattaacgat acaaaccaaa
361 cgcaacaaga acatttgtag tattatctat aattgaaaac gcgtagttat aatcgctgag
421 gtaatattta aaatcatttt caaatgattc acagttaatt tgcgacaata taattttatt
481 ttcacataaa ctagacgcct tgtcgtcttc ttcttcgtat tccttctctt tttcattttt
541 ctcttcataa aaattaacat agttattatc gtatccatat atgtatctat cgtatagagt
601 aaattttttg ttgtcataaa tatatatgtc ttttttaatg gggtgtatag taccgctgcg
661 catagttttt ctgtaattta caacagtgct attttctggt agttcttcgg agtgtgttgc
721 tttaattatt aaatttatat aatcaatgaa tttgggatcg tcggttttgt acaatatgtt
781 gccggcatag tacgcagctt cttctagttc aattacacca ttttttagca gcaccggatt
841 aacataactt tccaaaatgt tgtacgaacc gttaaacaaa aacagttcac ctcccttttc
901 tatactattg tctgcgagca gttgtttgtt gttaaaaata acagccattg taatgagacg
961 cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctga tggccggccg
1021 taatgagacg cacaaactaa tatcacaaac tggaaatgtc tatcaatata tagttgctct
1081 agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc
1141 gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg
1201 acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa
1261 tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca
1321 agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac
1381 atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc
1441 atgcatggtc gaggtgagcc ccacgttctg cttcactctc cccatctccc ccccctcccc
1501 acccccaatt ttgtatttat ttatttttta attattttgt gcagcgatgg gggcgggggg
1561 gggggggggg cgcgcgccag gcggggcggg gcggggcgag gggcggggcg gggcgaggcg
1621 gagaggtgcg gcggcagcca atcagagcgg cgcgctccga aagtttcctt ttatggcgag
1681 gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg cgggcgggag tcgctgcgcg
1741 ctgccttcgc cccgtgcccc gctccgccgc cgcctcgcgc cgcccgcccc ggctctgact
1801 gaccgcgtta ctcccacagg tgagcgggcg ggacggccct tctccttcgg gctgtaatta
1861 gcgcttggtt taatgacggc ttgtttcttt tctgtggctg cgtgaaagcc ttgaggggct
1921 ccgggagggc cctttgtgcg gggggagcgg ctcggggctg tccgcggggg gacggctgcc
1981 ttcggggggg acggggcagg gcggggttcg gcttctggcg tgtgaccggc ggctctagag
2041 cctctgctaa ccatgttcat gccttcttct ttttcctaca gctcctgggc aacgtgctgg
2101 ttattgtgct gtctcatcat tttggcaaag aattggatcg gaccgaaatt aatacgactc
2161 actatagggg aattgtgagc ggataacaat tccccggagt taatccggga cctttaattc
2221 aacccaacac aatatattat agttaaataa gaattattat caaatcattt gtatattaat
2281 taaaatacta tactgtaaat tacattttat ttacaatcaa aggagatata ccatggcgat
2341 atcccgggtg gatccgaatt ctcagatctc ggcgcgcctg cagggttcga agcttatact
2401 cgagaagcgt tgaaatagcg tacaagtcga gcaccaccat caccatcacc atcactaagt
2461 gattaacctc aggtgcaggc tgcctatcag aaggtggtgg ctggtgtggc caatgccctg
2521 gctcacaaat accactgaga tcgatctttt tccctctgcc aaaaattatg gggacatcat
2581 gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca ttgcaatagt
2641 gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa atcatttaaa
2701 acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgt aactagcata
2761 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaagcat gcggaggaaa
2821 ttctccttga agtttccctg gtgttcaaag taaaggagtt tgcaccagac gcacctctgt
2881 tcactggtcc ggcgtattaa aacacgatac attgttatta gtacatttat taagcgctag
2941 attctgtgcg ttgttgattt acagacaatt gttgtacgta ttttaataat tcattaaatt
3001 tataatcttt agggtggtat gttagagcga aaatcaaatg attttcagcg tctttatatc
3061 tgaatttaaa tattaaatcc tcaatagatt tgtaaaatag gtttcgatta gtttcaaaca
3121 agggttgttt ttccgaaccg atggctggac tatctaatgg attttcgctc aacgccacaa
3181 aacttgccaa atcttgtagc agcaatctag ctttgtcgat attcgtttgt gttttgtttt
3241 gtaataaagg ttcgacgtcg ttcaaaatat tatgcgcttt tgtatttctt tcatcactgt
3301 cgttagtgta caattgactc gacgtaaaca cgttaaatag agcttggaca tatttaacat
3361 cgggcgtgtt agctttatta ggccgattat cgtcgtcgtc ccaaccctcg tcgttagaag
3421 ttgcttccga agacgatttt gccatagcca cacgacgcct attaattgtg tcggctaaca
3481 cgtccgcgat caaatttgta gttgagcttt ttggaattgc gatcgcataa cttcgtatag
3541 catacattat acgaagttat aagctcggaa cgctgcgctc ggtcgttcgg ctgcggcgag
3601 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
3661 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
3721 tggcgttttt ccataggctc cgccccctga cgagcatcac aaaaatcgac gctcaagtca
3781 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
3841 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
3901 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
3961 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
4021 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
4081 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
4141 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
4201 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
4261 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
4321 tcctttgtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
4381 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
4441 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
4501 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
4561 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
4621 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
4681 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
4741 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
4801 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
4861 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
4921 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
4981 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
5041 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
5101 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
5161 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
5221 atcagggtta ttgtctcatg tccgcgcgtt
//