质粒序列
LOCUS Exported 2897 bp ds-DNA circular SYN 11-9-2015
DEFINITION .
ACCESSION .
VERSION .
KEYWORDS Untitled 2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 2897)
AUTHORS admin
TITLE Direct Submission
JOURNAL Exported 2015-9-11 from MLCC
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..2897
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 20..38
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 112..129
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
CDS 133..165
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/note="T7 tag (gene 10 leader)"
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
CDS 169..192
/codon_start=1
/product="Xpress(TM) epitope tag, including an enterokinase
recognition and cleavage site"
/note="Xpress(TM) tag"
/translation="DLYDDDDK"
terminator 312..359
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 456..911
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 937..1041
/gene="bla"
/note="AmpR promoter"
CDS 1042..1902
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2073..2661
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
61 agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat
121 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
181 gatgacgata aggatcgatg gggatccgag ctcgagatct gcagctggta ccatggaatt
241 cgaagcttga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg
301 ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc
361 tgaaaggagg aactatatcc ggatctggcg taatagcgaa gaggcccgca ccgatcgccc
421 ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag
481 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
541 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
601 tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
661 aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
721 ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
781 actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
841 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
901 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
961 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
1021 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
1081 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
1141 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
1201 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
1261 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
1321 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
1381 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
1441 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
1501 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
1561 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
1621 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
1681 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
1741 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
1801 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
1861 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
1921 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
1981 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
2041 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
2101 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
2161 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
2221 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
2281 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
2341 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
2401 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
2461 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
2521 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
2581 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
2641 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
2701 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
2761 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
2821 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
2881 ccgattcatt aatgcag
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