质粒序列
LOCUS Exported 5712 bp ds-DNA circular SYN 11-八月-2015
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5712)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 2015年8月11日 from SnapGene Viewer 2.8.0
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..5712
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 20..38
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 39..63
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 112..129
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
terminator 238..285
/note="T7 terminator"
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter 609..713
/gene="bla"
/note="AmpR promoter"
CDS 714..1574
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 1745..2333
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
misc_feature 2519..2658
/note="bom"
/note="basis of mobility region from pBR322"
CDS complement(2760..2951)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/note="rop"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
CDS complement(4263..5345)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/note="lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
promoter complement(5346..5423)
/gene="lacI"
/note="lacI promoter"
ORIGIN
1 gatctcgatc ccgcgaaatt aatacgactc actatagggg aattgtgagc ggataacaat
61 tcccctctag aaataatttt gtttaaactt taagaaggag atatacatat gcatcatcat
121 catcatcacg tgaattcgct cgagatcgat gatattcgag cctaggtata atcggatccg
181 gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta
241 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact
301 atatccggat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag
361 catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg
421 cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctag cttatcgatg
481 ataagctgtc aaacatgaga attaattctt gaagacgaaa gggcctcgtg atacgcctat
541 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
601 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
661 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
721 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
781 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
841 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
901 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg
961 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
1021 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
1081 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
1141 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
1201 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag
1261 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
1321 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
1381 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
1441 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
1501 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
1561 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
1621 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
1681 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
1741 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
1801 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
1861 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc
1921 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
1981 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
2041 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
2101 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
2161 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
2221 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
2281 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
2341 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
2401 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
2461 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
2521 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcaat ggtgcactct
2581 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt
2641 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct
2701 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt
2761 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg
2821 tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc
2881 tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc
2941 tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg
3001 atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg
3061 gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact
3121 cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag
3181 catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga
3241 ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg
3301 cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg
3361 caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc
3421 caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg
3481 gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct
3541 ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg
3601 tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc
3661 ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga
3721 tcagcggtcc aatgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc
3781 cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc
3841 cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca
3901 gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga
3961 aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata
4021 ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga
4081 cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg
4141 cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca
4201 agggcatcgg tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgttgc
4261 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
4321 aacgcgcggg gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt
4381 gagacgggca acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg
4441 tccacgctgg tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata
4501 taacatgagc tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc
4561 agcccggact cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc
4621 atcgcagtgg gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg
4681 gcactccagt cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta
4741 tgccagccag ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg
4801 atttgctggt gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg
4861 gagaaaataa tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca
4921 ttagtgcagg cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc
4981 agcccactga cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg
5041 cttcgttcta ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc
5101 gccgcgacaa tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc
5161 aacgactgtt tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc
5221 atcgccgctt ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg
5281 cgggaaacgg tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt
5341 ttcacattca ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag
5401 gttttgcgcc attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat
5461 taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc
5521 atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc
5581 gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc
5641 gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc
5701 gtagaggatc ga
//