pET-5b质粒是一种原核表达载体，含有一个N端的T7标签和BamHI限制性内切酶位点。表达由宿主细胞提供的T7 RNA聚合酶诱导，目的基因被克隆到质粒载体上，受噬菌体强转录及翻译信号控制。
pET系统是有史以来在大肠杆菌中表达重组蛋白的功能*强大的系统，也是现今原核表达方面使用*广泛的系统。该系列质粒能很容易的通过降低诱导物的浓度来削弱蛋白表达。在非诱导条件下，可以使目的基因处于完全沉默状态而不转录。
质粒序列
LOCUS Exported 4130 bp ds-DNA circular SYN 20-09-2016
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Untitled 2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4130)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported 2016-9-20 from SnapGene Viewer 2.8.1
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4130
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(12..44)
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/note="T7 tag (gene 10 leader)"
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
RBS 52..74
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
promoter complement(106..124)
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 1685..1876
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/note="rop"
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
misc_feature 1978..2117
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(2303..2891)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3062..3922)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(3923..4027)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 ttcggatccc gacccatttg ctgtccacca gtcatgctag ccatatgtat atctccttct
61 taaagttaaa caaaattatt tctagaggga aaccgttgtg gtctccctat agtgagtcgt
121 attaatttcg cgggatcgag atctcgatcc tctacgccgg acgcatcgtg gccggcatca
181 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc
241 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg
301 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc
361 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc
421 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
481 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
541 cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg
601 gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc
661 ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc
721 tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc
781 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
841 atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga
901 tcattggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt
961 tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg
1021 catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac
1081 cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc
1141 ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg
1201 cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct
1261 aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg
1321 aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt
1381 ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat
1441 ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg
1501 ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt
1561 accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat
1621 cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc
1681 atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac
1741 attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga
1801 atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga
1861 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga
1921 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc
1981 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca
2041 gagcagattg tactgagagt gcaccattgc ggtgtgaaat accgcacaga tgcgtaagga
2101 gaaaataccg catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
2161 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
2221 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
2281 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
2341 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
2401 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
2461 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
2521 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
2581 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
2641 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
2701 cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
2761 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
2821 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
2881 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
2941 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
3001 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
3061 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
3121 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
3181 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
3241 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
3301 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
3361 acgttgttgc cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
3421 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
3481 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
3541 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
3601 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
3661 gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc
3721 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
3781 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
3841 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
3901 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
3961 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
4021 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
4081 cattaaccta taaaaatagg cgtatcacga ggccctttcg tcttcaagaa
//