质粒序列
LOCUS Exported 4392 bp ds-DNA circular SYN 13-SEP-2016
DEFINITION synthetic circular DNA
KEYWORDS Untitled 4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4392)
TITLE Direct Submission
JOURNAL Exported Tuesday, September 13, 2016 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4392
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 84..100
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 271..288
/codon_start=1
/product="6xHis affinity tag"
/note="6xHis"
/translation="HHHHHH"
rep_origin complement(696..1151)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1251..1355
/gene="bla"
/note="AmpR promoter"
CDS 1356..2216
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2387..2975
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3147..4229)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/note="lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
promoter complement(4230..4307)
/gene="lacI"
/note="lacI promoter"
ORIGIN
1 aaggaatggt gtggccgatt aatcataaat atgaaaaata attgttgcat cacccgccaa
61 tgcgtggctt aatgcacatc aaattgtgag cggataacaa tttgatgtgc tagcgcatat
121 ccagtgtagt aaggcaagtc ccttcaagag ttatcgttga tacccctcgt agtgcacatt
181 cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc gaaaggcaca cttaattatt
241 aagaggtaat acaccatgaa tcacaaagtg catcatcatc atcatcatat ggagctcggt
301 accctcgagg gatccgaatt caagcttgtc gacctgcagt ctagataggt aatctctgct
361 taaaagcaca gaatctaaga tccctgccat ttggcgggga tttttttatt tgttttcagg
421 aaataaataa tcgatcgcgt aataaaatct attattattt ttgtgaagaa taaatttggg
481 tgcaatgaga atgcgcaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct
541 ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
601 acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc
661 ggcatcagag cagattgtac tgagagtgca ccataaaatt gtaaacgtta atattttgtt
721 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
781 caaaatccct tataaatcaa aagaatagcc cgagataggg ttgagtgttg ttccagtttg
841 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
901 tcagggcgat ggcccactac gtgaaccatc acccaaatca agttttttgg ggtcgaggtg
961 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
1021 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
1081 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
1141 acagggcgcg tactatggtt gctttgacgt atgcggtgtg aaataccgca cagatgcgta
1201 aggagaaaat accgcatcag gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
1261 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
1321 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
1381 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
1441 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
1501 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
1561 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
1621 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
1681 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
1741 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
1801 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
1861 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
1921 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
1981 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
2041 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
2101 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
2161 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
2221 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
2281 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
2341 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
2401 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
2461 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
2521 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
2581 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
2641 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
2701 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
2761 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
2821 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
2881 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
2941 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
3001 ggttcctggc cttttgctgg ccttttgctc acatagtcat gccccgcgcc caccggaagg
3061 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga
3121 gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
3181 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc
3241 agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg
3301 ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt
3361 ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact
3421 accgagatat ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc
3481 gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc
3541 atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga
3601 atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa
3661 cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg
3721 cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag
3781 acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg
3841 tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc
3901 gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc
3961 agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga
4021 ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg
4081 ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa
4141 acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct
4201 gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg
4261 cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg
4321 ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag
4381 caccgccgcc gc
//