质粒序列
LOCUS Exported 4359 bp ds-DNA circular SYN 13-SEP-2016
DEFINITION synthetic circular DNA
KEYWORDS Untitled 8
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4359)
TITLE Direct Submission
JOURNAL Exported Tuesday, September 13, 2016 from MiaoLingPlasmid
http://www.miaolingbio.com
FEATURES Location/Qualifiers
source 1..4359
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 84..100
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
rep_origin complement(663..1118)
/direction=LEFT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1218..1322
/gene="bla"
/note="AmpR promoter"
CDS 1323..2183
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2354..2942
/direction=RIGHT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3114..4196)
/codon_start=1
/gene="lacI"
/product="lac repressor"
/note="lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
promoter complement(4197..4274)
/gene="lacI"
/note="lacI promoter"
ORIGIN
1 aaggaatggt gtggccgatt aatcataaat atgaaaaata attgttgcat cacccgccaa
61 tgcgtggctt aatgcacatc aaattgtgag cggataacaa tttgatgtgc tagcgcatat
121 ccagtgtagt aaggcaagtc ccttcaagag ttatcgttga tacccctcgt agtgcacatt
181 cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc gaaaggcaca cttaattatt
241 aagaggtaat accatatgga gctcggtacc ctcgagggat ccgaattcaa gcttgtcgac
301 ctgcagtcta gataggtaat ctctgcttaa aagcacagaa tctaagatcc ctgccatttg
361 gcggggattt ttttatttgt tttcaggaaa taaataatcg atcgcgtaat aaaatctatt
421 attatttttg tgaagaataa atttgggtgc aatgagaatg cgcaggccct ttcgtctcgc
481 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
541 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
601 cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca
661 taaaattgta aacgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
721 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagcccga
781 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
841 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
901 caaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
961 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
1021 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
1081 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtac tatggttgct ttgacgtatg
1141 cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg tcaggtggca
1201 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
1261 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
1321 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
1381 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
1441 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
1501 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
1561 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
1621 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
1681 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
1741 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
1801 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
1861 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
1921 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
1981 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
2041 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
2101 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
2161 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
2221 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
2281 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
2341 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
2401 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
2461 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
2521 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
2581 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
2641 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
2701 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
2761 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
2821 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
2881 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
2941 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
3001 tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg
3061 gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc
3121 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg
3181 ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc
3241 aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg
3301 gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag
3361 ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg cagcccggac
3421 tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg
3481 ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag
3541 tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca
3601 gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg
3661 tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata
3721 atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag
3781 gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg
3841 acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct
3901 accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca
3961 atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt
4021 ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct
4081 tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg
4141 gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc
4201 accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc
4261 cattcgatgg tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaagca
4321 gcccagtagt aggttgaggc cgttgagcac cgccgccgc
//